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首页> 外文期刊>Journal of Clinical Microbiology >Investigation of the reformulated Remel Synergy Quad plate for detection of high-level aminoglycoside and vancomycin resistance among enterococci.
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Investigation of the reformulated Remel Synergy Quad plate for detection of high-level aminoglycoside and vancomycin resistance among enterococci.

机译：重新配制的Remel Synergy Quad板用于检测肠球菌中高水平氨基糖苷和万古霉素耐药性的研究。

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摘要

We investigated the accuracy of the recently released Remel Synergy Quad plate, a commercially available agar screening method for detecting high-level aminoglycoside and vancomycin resistance among enterococci that is based on the National Committee for Clinical Laboratory Standards recommended guidelines (National Committee for Clinical Laboratory Standards, M7-A3, 1993). The Synergy Quad correctly determined the gentamicin and streptomycin resistance status for > or = 97% of 147 Enterococcus faecalis and Enterococcus faecium isolates tested. Detection of vancomycin resistance also was reliable, as no false susceptibility occurred with 36 vancomycin-resistant E. faecalis and E. faecium strains and false resistance occurred only once with the 47 susceptible strains tested. One strain each of Enterococcus gallinarum and Enterococcus casseliflavus failed to grow on the screen, but because the true nature and significance of resistance in such isolates is unknown the implication of their screen negativity is uncertain. In summary, the Remel Synergy Quad provides a highly accurate and convenient method for susceptibility testing of enterococci against gentamicin, streptomycin, and vancomycin.
机译：我们调查了最近发布的Remel Synergy Quad板的准确性，Remel Synergy Quad板是一种可商购的琼脂筛选方法，用于检测肠球菌中的高水平氨基糖苷和万古霉素耐药性，该方法基于国家临床实验室标准委员会推荐的指导原则（国家临床实验室标准委员会，M7-A3，1993）。 Synergy Quad正确确定了147种粪肠球菌和粪肠球菌分离株中≥97％的庆大霉素和链霉素耐药状态。万古霉素耐药性的检测也是可靠的，因为在36株耐万古霉素的粪肠球菌和屎肠球菌菌株中没有发生假药敏性，而在47种易感菌株中仅出现了一次假性耐药性。鸡肠球菌和Casseliflavus肠球菌各有一种菌株无法在筛选条件下生长，但是由于此类菌株中抗药性的真正性质和重要性尚不明确，因此其筛选阴性的含义尚不确定。总之，Remel Synergy Quad为肠球菌对庆大霉素，链霉素和万古霉素的敏感性测试提供了一种高度准确和便捷的方法。

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