...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Amplicor, in-house PCR, and conventional culture for detection of Mycobacterium tuberculosis in clinical samples.
【24h】

Comparison of Amplicor, in-house PCR, and conventional culture for detection of Mycobacterium tuberculosis in clinical samples.

机译：比较Amplicor，内部PCR和常规培养以检测临床样品中的结核分枝杆菌。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Five hundred four clinical specimens (337 sputum and 167 bronchial samples) from 340 patients were tested for the presence of M. tuberculosis complex by the Amplicor M. tuberculosis test and by an in-house PCR. The results were compared with those obtained by conventional culture and by direct microscopy. Thirty specimens (from 14 patients) were positive by in-house PCR, 25 (from 13 patients) were positive by the Amplicor M. tuberculosis test, and 24 (from 10 patients) were positive by culture. Cultures from 16 specimens were contaminated with other bacteria. Strong inhibition of in-house PCR was found with three samples. After discordancy analyses, with clinical data as supportive evidence for tuberculosis, 27 true-positive and 458 true-negative samples were defined. On the basis of these figures, the sensitivities of the Amplicor M. tuberculosis test, in-house PCR, culture, and microscopy were 70.4, 92.6, 88.9, and 52.4%, respectively. The specificities of all four tests were higher than 98%. The good performance of the in-house PCR for detection of M. tuberculosis makes it a very useful additional tool in M. tuberculosis diagnostics. In contrast, the Amplicor test needs to be improved. Twenty-three of the Amplicor-negative samples were further tested for inhibition of the Amplicor system by retesting the DNA extracts after the addition of M. tuberculosis DNA. In 15 of these samples, 5 true positives and 10 true negatives, inhibition of the Amplicor test was demonstrated. This might explain the lack of sensitivity of the Amplicor test. If the inhibition problem can be solved, the Amplicor M. tuberculosis test, which is already rapid, very user-friendly, and reasonably priced, may certainly become very useful in microbiological laboratories.
机译：通过Amplicor结核分枝杆菌检测和内部PCR检测了340例患者的504个临床标本（337痰液和167支气管样本）。将结果与常规培养和直接显微镜观察的结果进行比较。内部PCR检测到30个样本（来自14例患者）为阳性，Amplicor结核分枝杆菌试验检测为25个（来自13例患者）为阳性，培养物检测为24个（来自10例患者）为阳性。来自16个标本的培养物被其他细菌污染。发现三个样品对内部PCR有很强的抑制作用。经过不一致分析后，以临床数据作为结核病的支持证据，定义了27个真阳性和458个真阴性样本。根据这些数据，Amplicor结核分枝杆菌试验，室内PCR，培养和显微镜检查的灵敏度分别为70.4％，92.6％，88.9％和52.4％。所有四个测试的特异性均高于98％。内部PCR检测结核分枝杆菌的良好性能使其成为结核分枝杆菌诊断中非常有用的附加工具。相反，Amplicor测试需要改进。在添加结核分枝杆菌DNA后，通过重新测试DNA提取物，进一步测试了23个Amplicor阴性样品对Amplicor系统的抑制作用。在这些样品中的15个，5个真阳性和10个真阴性中，证明了对Amplicor测试的抑制。这可能解释了Amplicor测试缺乏敏感性的原因。如果可以解决抑制问题，那么已经在快速，非常人性化且价格合理的Amplicor结核分枝杆菌试验肯定会在微生物实验室中变得非常有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号