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首页> 外文期刊>Journal of Clinical Microbiology >Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments.
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Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments.

机译：使用合成DNA片段通过竞争PCR对胃粘液中的幽门螺杆菌进行定量研究。

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摘要

Helicobacter pylori is closely related to upper gastrointestinal diseases, and the precise evaluation of H. pylori infection is necessary for the treatment of these diseases. The aim of the present study was to establish a method for the quantitative detection of H. pylori. We applied a competitive PCR method using various amounts of synthetic DNA fragments containing the same primer-binding and a subset of the same template sequences as the target competing for primer binding and amplification in order to quantify H. pylori in gastric mucus. The results obtained by this method were compared with the results of histological examination, the rapid urease test, bacterial culture, the [13C]urea breath test, and urea and ammonia measurements in gastric juice. As the quantity of H. pylori in gastric mucus increased, the rates of positivity of histological examination, the rapid urease test, and bacterial culture increased. The quantity of H. pylori in gastric mucus was also significantly correlated with the results of the [13C]urea breath test and was negatively correlated with the urea/ammonia ratio in gastric juice. The competitive PCR method provides an objective measure of the quantity of H. pylori and makes it possible to distinguish true negatives from false negatives due to incomplete PCR and true positives from false positives due to contamination. This method is very useful for the precise evaluation of gastric H. pylori infection.
机译：幽门螺杆菌与上消化道疾病密切相关，对幽门螺杆菌感染的精确评估对于治疗这些疾病是必要的。本研究的目的是建立一种定量检测幽门螺杆菌的方法。我们应用了竞争性PCR方法，使用了各种数量的合成DNA片段，这些片段包含与目标竞争相同的引物结合和扩增的相同引物结合和相同模板序列的子集，以便量化胃粘液中的幽门螺杆菌。通过此方法获得的结果与组织学检查，快速尿素酶测试，细菌培养，[13C]尿素呼气试验以及胃液中尿素和氨的测量结果进行了比较。随着胃黏液中幽门螺杆菌数量的增加，组织学检查，快速尿素酶检测和细菌培养的阳性率增加。胃粘液中幽门螺杆菌的数量也与[13C]尿素呼气试验的结果显着相关，与胃液中尿素/氨比呈负相关。竞争性PCR方法提供了一种幽门螺杆菌数量的客观测量方法，并可以区分由于PCR不完全导致的阴性阴性与阴性阴性以及由于污染导致的阳性阳性与阴性阳性之间的区别。该方法对于精确评估胃幽门螺杆菌感染非常有用。

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