...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Isolation of Bartonella (Rochalimaea) henselae: effects of methods of blood collection and handling.
【24h】

Isolation of Bartonella (Rochalimaea) henselae: effects of methods of blood collection and handling.

机译：亨氏巴尔通体的分离：采血和处理方法的影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bartonella (Rochalimaea) henselae causes cat-scratch disease, bacillary angiomatosis, peliosis hepatis, and fever in humans. B. henselae can be difficult to culture axenically, and as many as 5 weeks may be required before colonies are visible. We compared how different methods of blood collection and handling affect isolation of this pathogen. Blood specimens from B. henselae-infected cats were collected in both EDTA and Isolator blood-lysis tubes and were subsequently plated onto rabbit blood-brain heart infusion agar by using three different schedules: plating immediately, plating after 24 h at 25 degrees C, and plating after 26 days at -65 degrees C. Colonies were counted 14 and 35 days after plating. Blood collected in tubes containing EDTA, frozen at -65 degrees C, and then plated on blood agar yielded a median of 60,000 CFU/ml, compared with 25,333 CFU/ml after collection in the Isolator tubes (P < 0.01). Frozen blood yielded the largest number of B. henselae colonies for any of the schedules tested. These results support previous observations that the Isolator system is more sensitive than tubes containing EDTA for isolation of B. henselae and suggest that, for cat blood, collection in tubes containing EDTA and subsequent freezing may further improve the sensitivity of detection of B. henselae.
机译：巴尔通体（Rochalimaea）henselae会引起猫抓病，细菌性血管瘤病，肝性肝硬化和人类发烧。亨氏芽孢杆菌可能难以进行无菌培养，并且在可见菌落之前可能需要多达5周的时间。我们比较了不同的采血和处理方法如何影响这种病原体的分离。在EDTA和Isolator溶血试管中收集感染汉森双歧杆菌的猫的血样，然后按照以下三种时间表将其接种到兔血脑心脏琼脂上：立即铺板，在25摄氏度24小时后铺板，在-65℃下放置26天后进行铺板。在铺板之后14和35天计数菌落。在装有EDTA的试管中收集的血液在-65摄氏度下冷冻，然后铺在血琼脂上，其中位值为60,000 CFU / ml，而在隔离器试管中收集后为25,333 CFU / ml（P <0.01）。在任何测试时间表中，冷冻血液产生的汉森芽孢杆菌菌落数量最多。这些结果支持了以前的观察结果，即隔离器系统比含EDTA的试管对汉逊双歧杆菌的分离更为敏感，并表明，对于猫血，在含EDTA的试管中收集并随后冷冻可进一步提高检测双歧杆菌的灵敏度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号