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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Adenoviruses (AdV) in Culture-Negative Environmental Samples by PCR during an AdV-Associated Respiratory Disease Outbreak
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Detection of Adenoviruses (AdV) in Culture-Negative Environmental Samples by PCR during an AdV-Associated Respiratory Disease Outbreak

机译：在与AdV相关的呼吸道疾病暴发期间，通过PCR检测培养阴性环境样品中的腺病毒（AdV）。

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Since 1954, adenoviruses (AdV) have been recognized as an important cause of acute respiratory disease (ARD) among U.S. military recruits. Until recently, routine oral vaccination for AdV serotypes 4 and 7 eliminated epidemic AdV-associated ARD in this population. Now that the manufacturer has ceased production, vaccination has ended and AdV epidemics have reappeared. As part of a prospective epidemiological study during the high-risk ARD season, serial samples were obtained from ventilation system filters and tested for AdV by culture and PCR. An outbreak occurred during this surveillance. Of 59 air filters, 26 (44%) were AdV positive only by PCR. Sequence analysis confirmed the presence of AdV serotype 4, the implicated outbreak serotype. The number of AdV-related hospitalizations was directly correlated with the proportion of filters containing AdV; correlation coefficients were 0.86 (Pearson) and 0.90 (Spearman's rho). This is the first report describing a PCR method to detect airborne AdV during an ARD outbreak. It suggests that this technique can detect and quantify AdV-associated ARD exposure and may enable further definition of environmental effects on AdV-associated ARD spread.
机译：自1954年以来，腺病毒（AdV）被公认为是美军新兵急性呼吸道疾病（ARD）的重要原因。直到最近，常规的针对AdV血清型4和7的口服疫苗仍消除了该人群中与AdV相关的流行性ARD。现在制造商已经停止生产，疫苗接种已经结束，AdV流行病再次出现。作为高危ARD季节中前瞻性流行病学研究的一部分，从通风系统过滤器中获取了一系列样本，并通过培养和PCR测试了AdV。在此监视期间发生了爆发。在59个空气滤清器中，只有26个（44％）仅通过PCR获得AdV阳性。序列分析证实了AdV血清型4的存在，即涉及的暴发血清型。与AdV相关的住院次数与含有AdV的过滤器的比例直接相关。相关系数分别为0.86（皮尔逊）和0.90（斯皮尔曼的rho）。这是第一份描述在ARD暴发期间检测机载AdV的PCR方法的报告。这表明该技术可以检测和量化与AdV相关的ARD暴露，并且可以进一步定义环境对AdV相关的ARD传播的影响。

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