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首页> 外文期刊>Journal of Clinical Microbiology >Genetic Diversity of Streptococcus suis Strains Isolated from Pigs and Humans as Revealed by Pulsed-Field Gel Electrophoresis
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Genetic Diversity of Streptococcus suis Strains Isolated from Pigs and Humans as Revealed by Pulsed-Field Gel Electrophoresis

机译：从猪和人分离的猪链球菌菌株的遗传多样性通过脉冲场凝胶电泳显示

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摘要

The genetic diversity of 123 Streptococcus suis strains of capsular types 2, 1/2, 3, 7, and 9, isolated from pigs in France and from humans in different countries, was evaluated by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI. The method was highly discriminative (D = 0.98), results were reproducible, and the PFGE analysis was easy to interpret. Among all S. suis strains, 74 PFGE patterns were shown. At 60% homology, three groups (A, B, and C) were identified, and at 69% homology, eight subgroups (a to h) were observed. Strains isolated from diseased pigs or from humans were statistically clustered in group B, especially in subgroup d. By contrast, S. suis strains isolated from clinically healthy pigs were preferentially included in subgroup b of group A. Relationships could be established between capsular types 1/2, 3, and 9 and groups A, e, and B, respectively. S. suis strains isolated from humans were homogeneous, and a very high level of association between these strains and four DNA patterns was observed. The PFGE used in this study is a very useful tool for evaluating the genetic diversity of S. suis strains, and it would be used for epidemiological investigations.
机译：通过脉冲场凝胶法评估了从法国的猪和来自不同国家的人类分离的123种猪荚膜链球菌2、1 / 2、3、7和9型荚膜猪链球菌的遗传多样性。 Sma I限制的DNA的电泳（PFGE）。该方法具有很高的判别力（ D = 0.98），结果具有可重复性，并且PFGE分析易于解释。在所有 S中。 suis 菌株，显示74种PFGE模式。在60％的同源性下，确定了三组（A，B和C），在69％的同源性下，观察到了八个亚组（a至h）。从患病猪或人中分离出的菌株在B组中，特别是在D组中，统计上是聚类的。相比之下， S。从临床健康的猪中分离出的suis 菌株优先包括在A组的b组中。可以分别在1 / 2、3和9型荚膜荚膜与A，e和B组之间建立联系。 S。从人类分离出的suis 菌株是同质的，并且观察到这些菌株与四种DNA模式之间的关联程度很高。这项研究中使用的PFGE是评估 S遗传多样性的非常有用的工具。 suis 菌株，并将其用于流行病学调查。

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