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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis of the Pathogenicity Locus and Polymorphism in the Putative Negative Regulator of Toxin Production (TcdC) among Clostridium difficile Clinical Isolates
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Molecular Analysis of the Pathogenicity Locus and Polymorphism in the Putative Negative Regulator of Toxin Production (TcdC) among Clostridium difficile Clinical Isolates

机译：艰难梭菌临床分离株推定的毒素产生负调节剂（TcdC）致病性基因座和多态性的分子分析

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The pathogenicity locus (PaLoc) of Clostridium difficile contains toxin A and B genes and three accessory genes, including tcdD and tcdC, which are supposed to code for the positive and negative regulators of toxin expression, respectively. Different studies have described variations in C. difficile toxin A and B genes, but little is known about C. difficile variants for the accessory genes. The PaLoc of several C. difficile clinical isolates was investigated by three different PCR methods with the aim to identify variant strains. Of the toxinogenic C. difficile strains examined, 25% showed variations. No correlation between C. difficile variant strains and key patient groups was found. Interestingly, all of these strains showed a variant tcdC gene. Three different tcdC alleles were identified, and one of these had a nonsense mutation which reduced the TcdC protein from 232 to 61 amino acids. It is possible that different TcdC variants affect toxin production differently, a hypothesis with important implications for the pathogenic potential of variant C. difficile strains.
机译：艰难梭菌的致病性位点（PaLoc）包含毒素A和B基因以及三个辅助基因，包括 tcdD 和 tcdC 。分别表示毒素表达的正负调节剂。不同的研究描述了C的变化。艰难梭菌毒素A和B基因，但对 C知之甚少。辅助基因的艰难变种。几个 C的PaLoc。通过三种不同的PCR方法对难分离的临床分离株进行了研究，旨在鉴定出变异株。产毒素的。检查的艰难梭菌菌株中，有25％显示出变异。 C之间没有相关性。发现了艰难梭菌变异株和主要的患者群体。有趣的是，所有这些菌株都显示了一个变体 tcdC 基因。鉴定了三个不同的 tcdC 等位基因，其中一个具有无义突变，可将TcdC蛋白从232个氨基酸减少至61个氨基酸。不同的TcdC变体可能会不同地影响毒素产生，这一假设对变体C的致病潜力具有重要意义。艰难菌株。

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