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首页> 外文期刊>Journal of Clinical Microbiology >Distribution of the Intermedilysin Gene among the Anginosus Group Streptococci and Correlation between Intermedilysin Production and Deep-Seated Infection with Streptococcus intermedius
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Distribution of the Intermedilysin Gene among the Anginosus Group Streptococci and Correlation between Intermedilysin Production and Deep-Seated Infection with Streptococcus intermedius

机译：间质溶素基因在链球菌组中的分布及间质溶菌素的产生与中间链球菌深层感染的相关性

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摘要

The distribution of intermedilysin, a human-specific cytolysin, among the anginosus group streptococci and the correlation of toxin production and infection by Streptococcus intermedius were investigated. PCR and Southern hybridization specific for the intermedilysin gene revealed that the toxin gene exists only inS. intermedius and no homologue to the toxin gene is distributed in S. anginosus and S. constellatus. Thus, the intermedilysin gene is useful as a marker gene of S. intermedius. Moreover, a human-specific hemolysis assay and Western blotting with intermedilysin-specific antibodies clearly demonstrated that the intermedilysin production level in isolates from deep-seated infections, such as brain and liver abscesses, is higher (6.2- to 10.2-fold, respectively) than in strains from normal habitats, such as dental plaque, or from peripheral infection sites. However, other candidate virulence factors of S. intermedius, such as chondroitin sulfate depolymerase, hyaluronidase, and sialidase activities, did not show such a clear correlation between enzymatic activity and isolation sites or disease severity. From these results, intermedilysin is likely to be the pathogenic or triggering factor of significance in inducing deep-seated infections with S. intermedius.
机译：研究了人源性溶细胞溶素间质溶素在链球菌组中的分布以及间质链球菌毒素产生与感染的相关性。对中间溶素基因进行的PCR和Southern杂交表明，该毒素基因仅存在于emS中。 intermedius ，并且毒素基因的同源物没有分布在 S中。心绞痛和 S。星座。因此，中间溶素基因可用作 S的标记基因。 intermedius 。此外，人类特异性溶血分析和间质溶素特异性抗体的Western印迹清楚地表明，深部感染（如脑和肝脓肿）分离株中间质溶素的生产水平更高（分别为6.2到10.2倍）而不是来自正常栖息地（如牙菌斑）或周围感染部位的菌株。但是， S的其他候选毒力因子。间质，如硫酸软骨素解聚酶，透明质酸酶和唾液酸酶活性，在酶活性与分离部位或疾病严重程度之间没有明显的相关性。从这些结果来看，中间溶素可能是诱发 S深层感染的重要病原或触发因素。 intermedius 。

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