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首页> 外文期刊>Infection and immunity >Role of Catabolite Control Protein A in the Regulation of Intermedilysin Production by Streptococcus intermedius
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Role of Catabolite Control Protein A in the Regulation of Intermedilysin Production by Streptococcus intermedius

机译：分解代谢控制蛋白A在中间链球菌生产中间溶素过程中的作用

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Streptococcus intermedius is an opportunistic pathogen of humans that causes purulent infections, including brain and liver abscesses. This pathogen secretes a human-specific cytolysin, intermedilysin, which has been recognized as a major virulence factor. However, most of the expressional control mechanisms of ily are still unknown. To determine these mechanisms, we analyzed the nucleotide sequence of the ily promoter region. We found a highly homologous region to the catabolite-repressible element (cre) in the ily promoter region and observed a considerable decrease in the amount of secreted intermedilysin when cells were grown in a culture medium containing high concentrations of glucose/utilizable carbohydrates. Disruption of the ccpA gene, which encodes catabolite control protein A, did not induce catabolite repression of ily by glucose/utilizable carbohydrates. In cre mutants, catabolite repression of ily was partially restored, and purified catabolite control protein A bound to an oligonucleotide containing the cre consensus sequence in the ily promoter region. In addition, a prolonged lag phase and slower doubling time of the ccpA mutant cells were observed. Our data show that S. intermedius can modulate ily expression and growth rate through catabolite control protein A-mediated monitoring of the extracellular glucose/utilizable carbohydrate concentration.
机译：中间链球菌是人类的机会性病原体，可引起化脓性感染，包括脑和肝脓肿。该病原体分泌人特异性细胞溶素，中间溶素，已被认为是主要的毒力因子。但是， ily 的大多数表达控制机制仍然未知。为了确定这些机制，我们分析了 ily 启动子区域的核苷酸序列。我们在 ily 启动子区域发现了与分解代谢物可抑制元件（ cre ）高度同源的区域，并观察到当细胞在含有高浓度葡萄糖/可利用碳水化合物的培养基。编码分解代谢物控制蛋白A的 ccpA 基因的破坏不会诱导葡萄糖/可利用碳水化合物对分解代谢物 ily 的抑制。在 cre 突变体中， ily 的分解代谢物阻遏被部分恢复，并且纯化的分解代谢物控制蛋白A与包含 cre 共有序列的寡核苷酸结合。 ily 启动子区域。此外，观察到 ccpA 突变细胞的延迟期延长和倍增时间变慢。我们的数据显示 S。 intermedius 可以通过分解代谢物控制蛋白A介导的细胞外葡萄糖/可利用碳水化合物浓度监测来调节 ily 的表达和生长速度。

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