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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis
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Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis

机译:人类粒细胞性毛虫病病因的分子分型

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The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII,EcoRV, and AspI to generate restriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GE typing method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae in the United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool.
机译:人类粒细胞埃希氏菌病(aoHGE)的 p44 基因编码一个44 kDa的主要外表面蛋白。根据 p44 基因的PCR扩增,然后用 Hin dIII, Eco RV和 Asp I生成限制性片段长度多态性模式。从美国地理上分散的地点收集了24个aoHGE样本,其中包括人类,马,犬,小型哺乳动物和tick的分离株。确定了六种粒细胞埃希氏菌病(GE)类型。 GE分型方法执行起来相对简单,可重现,并且能够在美国区分各种粒状大肠杆菌。这些特征表明,这种GE分型方法可能是重要的流行病学和流行病学工具。

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