...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Shiga Toxin-Producing Escherichia coli Infection and Antibodies against Stx2 and Stx1 in Household Contacts of Children with Enteropathic Hemolytic-Uremic Syndrome
【24h】

Shiga Toxin-Producing Escherichia coli Infection and Antibodies against Stx2 and Stx1 in Household Contacts of Children with Enteropathic Hemolytic-Uremic Syndrome

机译：志贺毒素生产性大肠埃希菌溶血性尿毒症综合征儿童家庭接触中的大肠杆菌感染和抗Stx2和Stx1抗体。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ninety-five household contacts (aged 2 months to 73 years) of patients with enteropathic hemolytic-uremic syndrome (HUS) were investigated for the presence of immunoglobulin (Ig) G antibodies to Shiga toxins Stx2 and Stx1 by Western blot assay. Thirty-one percent of the household contacts and 19% of 327 controls had anti-Stx2 IgG (heavy and light chain [H + L]), 5 and 8%, respectively, had anti-Stx1 IgG (H + L), and 3 and 2%, respectively, had both anti-Stx2 and anti-Stx1 IgG (H + L). The incidence of infections with Stx-producing Escherichia coli (STEC) was determined based on the following diagnostic criteria: STEC isolation, detection of stx gene sequences, free fecal Stx in stool filtrates, and serum IgM antibodies against E. coli O157 lipopolysaccharide. Evidence of STEC infection was observed in 25 household contacts, of whom 18 (72%) were asymptomatic and represented a potential source of infection. Six of 13 (46%) household contacts with Stx2-producing E. coli O157:H7 in stool culture developed anti-Stx2 IgG (H + L), compared to 71% of Stx2-associated HUS cases. In individuals showing anti-Stx2 IgG (H + L), the antibody response was directed against the B subunit in 69% of household contacts and 71% of controls, in contrast to 28% of HUS patients. In this investigation controls had a significant increase of the median of IgM antibodies to O157 lipopolysaccharide (LPS) with age, up to the fifth decade. The lack of disease in household contacts with B subunit-specific antibodies, as well as the significantly higher median of anti-O157 LPS IgM antibodies in controls beyond 4.9 years of age, suggests a protective role for anti-Stx and anti-O157 LPS antibodies.
机译：通过Western印迹分析调查了九十五个家庭接触者（年龄在2个月至73岁之间）的肠病性溶血性尿毒症综合征（HUS），以检查是否存在针对志贺毒素Stx2和Stx1的免疫球蛋白（Ig）G抗体。 327名对照中有31％的家庭接触者和19％的对照具有抗Stx2 IgG（重链和轻链[H + L]），分别有5％和8％具有抗Stx1 IgG（H + L），分别有3％和2％的人同时具有抗Stx2和抗Stx1 IgG（H + L）。根据以下诊断标准，确定产生Stx的大肠杆菌的感染发生率：STEC分离，检测 stx 基因序列，粪便中游离粪便Stx滤液和抗 E的血清IgM抗体。大肠杆菌O157脂多糖。在25个家庭接触者中观察到STEC感染的证据，其中18例（72％）无症状，是潜在的感染源。 13个家庭中有6个（46％）与生产Stx2的 E接触。粪便培养物中的大肠杆菌O157：H7产生了抗Stx2 IgG（H + L），而与Stx2相关的HUS病例为71％。在显示抗Stx2 IgG（H + L）的个体中，抗体反应针对69％的家庭接触者和71％的对照中的B亚基，而HUS患者的这一比例为28％。在这项研究中，直到第五个十年，随着年龄的增长，对照组的O157脂多糖（LPS）IgM抗体的中位数显着增加。与B亚基特异性抗体的家庭接触缺乏疾病，以及超过4.9岁的对照组中抗O157 LPS IgM抗体的中位数明显更高，这表明抗Stx和抗O157 LPS抗体具有保护作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号