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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Mycology Laboratory Proficiency Testing
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Evaluation of Mycology Laboratory Proficiency Testing

机译：真菌学实验室能力测试的评估

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Changes over the last decade in overt proficiency testing (OPT) regulations have been ostensibly directed at improving laboratory performance on patient samples. However, the overt (unblinded) format of the tests and regulatory penalties associated with incorrect values allow and encourage laboratorians to take extra precautions with OPT analytes. As a result OPT may measure optimal laboratory performance instead of the intended target of typical performance attained during routine patient testing. This study addresses this issue by evaluating medical mycology OPT and comparing its fungal specimen identification error rates to those obtained in a covert (blinded) proficiency testing (CPT) program. Identifications from 188 laboratories participating in the New York State mycology OPT from 1982 to 1994 were compared with the identifications of the same fungi recovered from patient specimens in 1989 and 1994 as part of the routine procedures of 88 of these laboratories. The consistency in the identification of OPT specimens was sufficient to make accurate predictions of OPT error rates. However, while the error rates in OPT and CPT were similar for Candida albicans, significantly higher error rates were found in CPT forCandida tropicalis, Candida glabrata, and other common pathogenic fungi. These differences may, in part, be due to OPT’s use of ideal organism representatives cultured under optimum growth conditions. This difference, as well as the organism-dependent error rate differences, reflects the limitations of OPT as a means of assessing the quality of routine laboratory performance in medical mycology.
机译：过去十年来，公开水平测试（OPT）法规的变化表面上旨在改善患者样品的实验室性能。但是，公开的（非盲目）测试格式和与错误值相关的监管处罚允许并鼓励实验室医生对OPT分析物采取额外的预防措施。结果，OPT可以测量最佳的实验室性能，而不是常规的患者测试期间达到的典型性能的预期目标。这项研究通过评估医学真菌学OPT并将其真菌标本识别错误率与隐蔽（盲）能力测试（CPT）程序中获得的真菌标本识别错误率进行比较，从而解决了这一问题。将1982年至1994年参加纽约州真菌学OPT的188个实验室的鉴定结果与1989和1994年从患者标本中回收的相同真菌的鉴定结果进行比较，作为这些实验室88个实验室常规程序的一部分。 OPT标本鉴定的一致性足以做出OPT错误率的准确预测。然而，尽管白色假丝酵母的OPT和CPT的错误率相似，但热带假丝酵母，光滑的假丝酵母的CPT的错误率却明显更高。 >，以及其他常见的致病真菌。这些差异可能部分是由于OPT使用了在最佳生长条件下培养的理想生物代表所致。这种差异以及与生物有关的错误率差异反映了OPT的局限性，它是评估医学真菌学中常规实验室表现质量的一种手段。

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