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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the Affirm Ambient Temperature Transport System for the Detection and Identification ofTrichomonas vaginalis, Gardnerella vaginalis, andCandida Species from Vaginal Fluid Specimens
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Evaluation of the Affirm Ambient Temperature Transport System for the Detection and Identification ofTrichomonas vaginalis, Gardnerella vaginalis, andCandida Species from Vaginal Fluid Specimens

机译：肯定性环境温度传输系统评估，用于从阴道液标本中检测和鉴定阴道毛滴虫，阴道加德纳菌和念珠菌

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The objective of this study was to measure the performance of the Affirm Ambient Temperature Transport System (ATTS) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis:Trichomonas vaginalis, Candida species, and Gardnerella vaginalis (one of the causative agents of bacterial vaginosis). Women with symptoms of vaginitis presenting to one of three clinical centers were evaluated over a 4- to 8-week period. Four simultaneously obtained swabs were collected and tested by the Affirm VPIII assay at time zero with and without a preservative reagent, at 24 h with reagent, and at either 48 or 72 h with reagent. For each of the three organisms,Trichomonas, Gardnerella, andCandida, positivity at each time point was evaluated and compared to that at reference time zero with and without the ATTS. A total of 940 specimens were obtained from the three clinical sites. Eight hundred three were positive for one or more of the three organisms. Gardnerella had the highest overall positive rate (62%), followed by Candida with 18% andTrichomonas at 9%. The percent sensitivity versus control for Trichomonas ranged from 100% at time zero with and without reagent to 91% by 72 h. Gardnerella andCandida sensitivity remained at 100% for each time period. The Affirm VPIII ATTS system performed within 10% of the control swab (no transport reagent) at all four time points (0, 24, 48, and 72 h) for Trichomonas, Gardnerella, andCandida.
机译：这项研究的目的是测量随时间变化的肯定环境温度传输系统（ATTS）的性能，并估计该系统可以保存包含三种引起阴道炎的常见生物的阴道标本的时间长度：阴道毛滴虫< / em>，念珠菌物种和阴道加德纳菌（细菌性阴道病的病原体之一）。在4到8周的时间内对出现在三个临床中心之一的患有阴道炎症状的妇女进行了评估。收集四个同时获得的拭子，并在零时间使用和不使用防腐剂的情况下，在使用试剂的24小时以及在使用试剂的48或72小时，通过Affirm VPIII分析进行测试。对于滴虫，加德纳菌和 Candida 这三种生物，分别评估每个时间点的阳性率，并将其与参考时间零比较有和没有ATTS。从三个临床地点总共获得了940个标本。三种生物中的一种或多种生物的八百零三阳性。 Gardnerella 的总体阳性率最高（62％），其次是 Candida 的18％和 Trichomonas 的9％。毛滴虫的相对于对照的敏感性百分比范围从零时有无试剂到零时的100％到72小时时为91％。在每个时间段内，和 Candida 的敏感性均保持在100％。对于滴虫， Gardnerella 的所有四个时间点（0、24、48和72小时），Affirm VPIII ATTS系统在对照拭子（无转运试剂）的10％内执行em>和 Candida 。

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