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首页> 外文期刊>Journal of Clinical Microbiology >Genetic Diversity of the Outer Surface Protein C Gene of Southern Borrelia Isolates and Its Possible Epidemiological, Clinical, and Pathogenetic Implications
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Genetic Diversity of the Outer Surface Protein C Gene of Southern Borrelia Isolates and Its Possible Epidemiological, Clinical, and Pathogenetic Implications

机译：南方疏螺旋体分离株的外表面蛋白C基因的遗传多样性及其可能的流行病学，临床和致病意义

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The ospC genes of 20 southern Borrelia strains were sequenced. The strains consisted of B. burgdorferi sensu stricto, B. andersonii, B. bissettii, one undescribed genospecies, MI-8, and one probably new Borrelia species, TXW-1. A high degree of similarity exists between B. burgdorferi sensu stricto and B. bissettii and between B. bissettii and B. andersonii. Lateral transfers of the ospC gene probably occurred between B. burgdorferi sensu stricto and B. bissettii and between B. bissettii and B. andersonii. Internal gene recombination appears to occur among them. The highest degree of genetic diversity among them was observed in the two variable domains (V1 and V2), semivariable domain (SV), and the species-specific epitopes (between amino acids 28 and 31). Differences in ospC sequences among southern strains reflect diversity at the strain and genospecies levels. MI-8, which was recognized as an undescribed genospecies in our previous reports, remains distinguishable in our current analysis of ospC genes and is distinct from B. burgdorferi sensu stricto. Interestingly, another undescribed southern isolate, TXW-1, was not amplified under various PCR conditions. Compared to European B. burgdorferi sensu stricto strains, American B. burgdorferi sensu stricto strains show greater genetic heterogeneity. Southern B. burgdorferi sensu stricto, B. andersonii, and B. bissettii isolates were intermixed with each other in the phylogenetic trees. In the derived trees in our work, at least one southeastern strain of B. burgdorferi, MI-2, most closely aligns with a so-called invasive cluster that possesses many proven human-invasive strains. Transmission experiments show that MI-2 and the strains in this group of southern spirochetes are able to infect mice and hamsters and that the typical vector of Lyme disease, Ixodes scapularis, can acquire the spirochetes from infected mammals. Currently, strain MI-2 appears to be the only southern isolate among the 20 we analyzed that clusters with an OspC invasive group and thus might be invasive for humans.
机译：测序了20个南部 Borrelia 菌株的 ospC 基因。菌株由 B组成。 burgdorferi sensu stricto， B。 Andersonii ， B。 bissettii ，一种未描述的基因种MI-8和一种可能是新的'Borrelia 物种TXW-1。 B之间存在高度相似性。 burgdorferi sensu stricto和 B。 bissettii 和 B之间。 bissettii 和 B。安德森二世。 ospC 基因的横向转移可能发生在 B之间。 burgdorferi sensu stricto和 B。 bissettii 和 B之间。 bissettii 和 B。安德森二世。内部基因重组似乎在其中发生。在两个可变域（V1和V2），半可变域（SV）和物种特异性表位（在氨基酸28和31之间）中观察到了其中最高程度的遗传多样性。南部菌株之间 ospC 序列的差异反映了菌株和基因物种水平的多样性。 MI-8，在我们先前的报告中被认为是未描述的基因种，在我们目前对 ospC 基因的分析中仍然可以区分，并且不同于 B。 burgdorferi sensustricto。有趣的是，另一个未描述的南方分离株TXW-1在各种PCR条件下均未扩增。与欧洲 B相比。 burgdorferi sensu stricto菌株，美国 B。 burgburgeri sensu stricto菌株显示出更大的遗传异质性。南部 B。 burgdorferi sensu stricto， B。 andersonii 和 B。将bissettii 菌株在系统发育树中相互混合。在我们工作的衍生树木中，至少有一种东南芽 B。 burgdorferi （MI-2）与拥有许多已证明的人类入侵菌株的所谓入侵簇最接近。传播实验表明，MI-2和该类南部螺旋体中的菌株能够感染小鼠和仓鼠，并且莱姆病的典型载体 scapularis 可以从被感染的哺乳动物中获得螺旋体。目前，MI-2菌株似乎是我们分析的20个带有OspC侵袭基团的簇中唯一的南方分离株，因此可能对人类具有侵袭性。

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