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首页> 外文期刊>Journal of Clinical Microbiology >Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni
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Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni

机译：多基因座序列分型作为流行病学工具调查空肠弯曲杆菌引起的胃肠炎暴发的实用程序

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Multilocus sequence typing (MLST) has been proven useful for the study of the global population structure of Campylobacter jejuni; however, its usefulness for the investigation of outbreaks of disease caused by C. jejuni has not been proven. In this study, MLST plus sequencing of the flaA short variable region (SVR) were applied to 47 isolates from 12 outbreaks of C. jejuni infection whose relatedness has been determined previously, and the results were compared to those of serotyping and pulsed-field gel electrophoresis (PFGE). Isolates implicated in an outbreak were indistinguishable by all four subtyping methods, with sporadic isolates being distinguished from outbreak isolates. Two sporadic isolates from one outbreak were resistant to SmaI digestion and therefore nontypeable by PFGE but were differentiated from the outbreak strain by the other methods. PFGE and flaA SVR typing were the most discriminatory methods, with discriminatory indices (DI) of 0.930 and 0.923, respectively. However, an epidemic strain from one outbreak was distinguished from the other outbreak isolates by flaA SVR typing; its flaA allele was different at five nucleotides, suggesting that this change was possibly mediated by recombination. MLST was less discriminatory than PFGE and flaA SVR typing (DI = 0.859), and many of the epidemic strains possessed common sequence types (STs) including ST-8, -21, -22, and -42. However, further discrimination within STs was achieved by flaA SVR typing or PFGE. The results from this study demonstrate that a combined approach of MLST plus flaA SVR typing provides a level of discrimination equivalent to PFGE for outbreak investigations.
机译：已经证明多基因座序列分型（MLST）可用于研究空肠弯曲杆菌的全球种群结构。但是，它对于调查由C引起的疾病暴发很有用。空肠尚未得到证实。在这项研究中，MLST加上 flaA 短可变区（SVR）的测序被应用于来自12个 C暴发的47个分离株。空肠感染的相关性已事先确定，并将结果与​​血清分型和脉冲场凝胶电泳（PFGE）进行了比较。四种亚型分型方法均无法区分与爆发相关的分离株，而零星分离株与爆发分离株则有所区别。一次暴发中的两个零星分离株对 Sma I消化具有抗性，因此不能通过PFGE进行分型，但可以通过其他方法与暴发菌株区分开。 PFGE和 flaA SVR分型是最具有鉴别力的方法，鉴别指数（DI）分别为0.930和0.923。但是，通过 flaA SVR分型将一次疫情的流行株与其他疫情分离株区分开。它的 flaA 等位基因在五个核苷酸处不同，这表明这种变化可能是由重组介导的。 MLST的区分性不如PFGE和 flaA SVR分型（DI = 0.859），并且许多流行株具有常见的序列类型（ST），包括ST-8，-21，-22和-42 。但是，通过 flaA SVR分型或PFGE可以实现ST内的进一步区分。这项研究的结果表明，MLST加上 flaA SVR的组合方法可以为爆发调查提供与PFGE相当的辨别水平。

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