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首页> 外文期刊>Journal of Clinical Microbiology >Using Major Outer Membrane Protein Typing as an Epidemiological Tool To Investigate Outbreaks Caused by Milk-Borne Campylobacter jejuni Isolates in California
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Using Major Outer Membrane Protein Typing as an Epidemiological Tool To Investigate Outbreaks Caused by Milk-Borne Campylobacter jejuni Isolates in California

机译：使用主要的外膜蛋白分型作为流行病学工具来调查由牛奶空肠弯曲杆菌空肠分离株引起的暴发

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We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7%) bovine fecal, 12 of 20 (60%) flush alley water, and 1 of 20 (5%) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6%) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.
机译：我们描述了使用主要的外膜蛋白（MOMP）类型作为筛查，比较人粪便中临床暴发菌株的空肠弯曲菌porA基因序列与两次牛奶传播期间分离的奶牛场菌株的 porA 序列加州的弯曲杆菌病爆发调查。通过多位点序列分型和脉冲场凝胶电泳分析证实了具有相同或紧密相关的 porA 序列的临床和环境菌株的遗传相关性。首次爆发涉及11个州教养所中的1,644例空肠弯曲杆菌感染，并与中央山谷监狱中的现场乳制品（奶店A）提供的巴氏杀菌牛奶消费有关。第二次爆发涉及8例确诊的空肠弯曲杆菌和3例可疑空肠弯曲杆菌病例，这些病例与在另一处中央山谷的乳制品场（乳场B）消费商业原料奶和原料巧克力初乳有关。两家奶牛场都在农场装瓶装液态奶，并将成品分发到异地。总共，从乳制品A上收集的15份（46.7％）牛粪中的7份，20份（60％）的冲洗胡同水中的12份和20份（5％）泻湖样品中的1份分离了空肠弯曲杆菌。从26个牛粪样本中的9个（占34.6％）培养空肠。在五个冲洗胡同水样本（乳品A）和四个牛粪便样本（乳品B）中发现了与临床暴发菌株没有区别的环境菌株。研究结果表明，MOMP分型是在进行劳动密集型分子分型方法之前对环境分离物进行分类的有用工具。

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