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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from a Korean Nationwide Survey
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Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from a Korean Nationwide Survey

机译：从韩国全国性调查中肺炎克雷伯菌和大肠埃希菌的临床分离株产生的广谱β-内酰胺酶的分子表征

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To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.
机译：为了确定肺炎克雷伯氏菌和大肠杆菌的临床分离株中广谱β-内酰胺酶（ESBLs）的流行和基因型，我们进行了抗生素敏感性测试，pI测定，诱导测试，转缀合和DNA测序分析。从韩国13所大学医院收集的509株分离株中，有39.2％产生了ESBL。在韩国每个地区都发现了产生ESBL的分离株。共有44.6％的分离株同时产生TEM型和SHV型ESBL，而52％的ESBL分离株通过转结合转移了对头孢他啶的抗性。 ESBL是TEM-19，TEM-20，TEM-52，SHV-2a，SHV-12，以及在韩国首次发现的一个新变体TEM-116。到目前为止，TEM-1和SHV-12是最常见的变体。 TEM-1，TEM-116和SHV-12在 K中显示很高的患病率。肺炎。两种分离株（大肠杆菌 SH16和肺炎克雷伯氏菌 SV3）产生了CMY-1类β-内酰胺酶，它们在抵抗头孢西丁和头孢替坦方面起决定性作用，例如以及TEM型酶（分别为TEM-20和TEM-52）。利用MIC模式和DNA测序分析，我们推测了韩国TEM型β-内酰胺酶中可能的进化方案：从TEM-1到TEM-19，从TEM-19到TEM-20，从TEM-20到TEM- 52。

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