Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis

Terri H. Hardwick; Brian Plikaytis; Pamela K. Cassiday; Gary Cage; Mark S. Peppler; Deborah Shea

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Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis

机译：百日咳博德特氏菌基因组DNA片段的重现性通过XbaI限制产生并通过脉冲场凝胶电泳解析

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The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified.

机译：评估了每个DNA片段在三个脉冲场凝胶电泳（PFGE）谱中的分子大小测量值的实验室内和实验室间变异性，以及三个百日咳百日咳杆菌的全PFGE谱的可重复性。株。实验室内变异的主要来源发生在传代培养之间，而不是凝胶之间或凝胶之间。每个PFGE谱都是可重复生成的，并由其复合片段的分子大小客观地定义。确定了最适合用作内部参考标准的菌株或概况。

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《Journal of Clinical Microbiology》 |2002年第3期|共6页
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Terri H. Hardwick; Brian Plikaytis; Pamela K. Cassiday; Gary Cage; Mark S. Peppler; Deborah Shea;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis [J] . Terri H. Hardwick, Brian Plikaytis, Pamela K. Cassiday, Journal of Clinical Microbiology . 2002,第3期

机译：百日咳博德特氏菌基因组DNA片段的重现性通过XbaI限制产生并通过脉冲场凝胶电泳解析
2. Use of restriction fragment length polymorphisms resolved by pulsed-field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes. [J] . J W Coffin, C Condon, C A Compston, Journal of Clinical Microbiology . 1992,第7期

机译：通过脉冲场凝胶电泳解析的限制性片段长度多态性用于鸟分枝杆菌复合物中分枝杆菌的亚种鉴定和DNA探针的分离。
3. Use of restriction fragment length polymorphisms resolved by pulsed-field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes. [J] . J W Coffin, C Condon, C A Compston, Journal of Clinical Microbiology . 1992,第7期

机译：通过脉冲场凝胶电泳解析的限制性片段长度多态性用于鸟分枝杆菌复合物中分枝杆菌的亚种鉴定和DNA探针的分离。
4. COMPARISON OF AN AUTOMATED RIBOTYPING SYSTEM, PULSED-FIELD GEL ELECTROPHORESIS AND RANDOMLY AMPLIFIED POLYMORPHIC DNA FINGERPRINTING FOR GENOTYPING STREPTOCOCCUS UBERIS [C] . B.E. Gillespie, S.P. Oliver Annual Meeting of the National Mastitis Council . 2003

机译：基因分型链球菌的自动化玻璃型系统，脉冲场凝胶电泳和随机扩增多态性DNA指纹纹理的比较
5. Analysis of near-infrared dye -labeled Sanger sequencing fragments with gel electrophoresis using time-resolved fluorescence lifetime identification methods. [D] . Lassiter, Suzanne Jeanel. 2001

机译：使用时间分辨荧光寿命鉴定方法，通过凝胶电泳分析近红外染料标记的Sanger测序片段。
6. Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis [O] . Terri H. Hardwick, Brian Plikaytis, Pamela K. Cassiday, 2002

机译：百日咳博德特氏菌基因组DNA片段的重现性通过XbaI限制产生并通过脉冲场凝胶电泳解析
7. Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis [O] . Hardwick, Terri H., Plikaytis, Brian, Cassiday, Pamela K., 2002

机译：百日咳博德特氏菌基因组DNA片段的重现性，通过XbaI限制产生并通过脉冲场凝胶电泳解析
8. Analysis by Pulsed-Field Gel Electrophoresis of DNA Double-Strand Breakage andRepair in Deinococcus Radiodurans and a Radiosensitive Mutant [R] . Grimsley, J. K., Masters, C. I., Clark, E. P., 1991

机译：脉冲场凝胶电泳分析DNa双链断裂和修复耐辐射球菌和放射敏感性突变体

Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis

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