...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Encapsulated and Noncapsulated Haemophilus influenzae and Determination of Phylogenetic Relationships by Multilocus Sequence Typing
【24h】

Characterization of Encapsulated and Noncapsulated Haemophilus influenzae and Determination of Phylogenetic Relationships by Multilocus Sequence Typing

机译：封装和非封装流感嗜血杆菌的鉴定和系统发育关系的多基因座序列分型

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A multilocus sequence typing (MLST) scheme has been developed for the unambiguous characterization of encapsulated and noncapsulated Haemophilus influenzae isolates. The sequences of internal fragments of seven housekeeping genes were determined for 131 isolates, comprising a diverse set of 104 serotype a, b, c, d, e, and f isolates and 27 noncapsulated isolates. Many of the encapsulated isolates had previously been characterized by multilocus enzyme electrophoresis (MLEE), and the validity of the MLST scheme was established by the very similar clustering of isolates obtained by these methods. Isolates of serotypes c, d, e, and f formed monophyletic groups on a dendrogram constructed from the differences in the allelic profiles of the isolates, whereas there were highly divergent lineages of both serotype a and b isolates. Noncapsulated isolates were distinct from encapsulated isolates and, with one exception, were within two highly divergent clusters. The relationships between the major lineages of encapsulated H. influenzae inferred from MLEE data could not be discerned on a dendrogram constructed from differences in the allelic profiles, but were apparent on a tree reconstructed from the concatenated nucleotide sequences. Recombination has not therefore completely eliminated phylogenetic signal, and in support of this, for encapsulated isolates, there was significant congruence between many of the trees reconstructed from the sequences of the seven individual loci. Congruence was less apparent for noncapsulated isolates, suggesting that the impact of recombination is greater among noncapsulated than encapsulated isolates. The H. influenzae MLST scheme is available at www.mlst.net, it allows any isolate to be compared with those in the MLST database, and (for encapsulated isolates) it assigns isolates to their phylogenetic lineage, via the Internet.
机译：已开发出一种多位点序列分型（MLST）方案，用于对封装和未封装的流感嗜血杆菌分离株进行明确的表征。确定了131个分离株的七个管家基因的内部片段序列，这些分离株包括104种血清型a，b，c，d，e和f分离株和27种未包囊的分离株。以前，许多封装的分离物已经通过多基因座酶电泳（MLEE）进行了表征，并且通过这些方法获得的分离物非常相似的聚类，确定了MLST方案的有效性。血清型c，d，e和f的分离株在树状图上形成了单系群，这是由分离株等位基因谱的差异构成的，而血清型a和b的分离株却存在高度分歧的谱系。未封装的分离株不同于封装的分离株，除了一个例外，它们位于两个高度不同的簇中。封装的 H的主要谱系之间的关系。从MLEE数据推断出的流感在等位基因图谱差异所构成的树状图上无法辨别，但在由级联核苷酸序列重建的树上很明显。因此，重组并没有完全消除系统发生信号，为此，对于封装的分离株，从七个单独基因座的序列重建的许多树木之间存在显着的一致性。非封装分离株的一致性不太明显，这表明非封装分离株之间重组的影响更大。 H。流感MLST方案可从www.mlst.net获得，它允许将任何分离株与MLST数据库中的分离株进行比较，并且（对于封装的分离株）它可以通过Internet将分离株分配到其系统谱系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号