Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

Haruyuki Hirata; Xuenan Xuan; Naoaki Yokoyama; Yoshifumi Nishikawa; Kozo Fujisaki; Naoyoshi Suzuki

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Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

机译：马鞭毛虫巴氏杆菌P82蛋白特定抗原区的鉴定及其在血清学诊断中的潜在用途

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The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported previously (H. Hirata et al., J. Clin. Microbiol. 40:1470-1474, 2002). However, the ELISA with the GST/Be82 antigen cross-reacted with Babesia caballi-infected horse sera, despite the high rate of detection of B. equi. These results suggested that GST/Be82 has an antigen in common with B. caballi or antigenicity similar to that of B. caballi. In the present study, we constructed a series of five clones with deletions in the Be82 gene product, each of which was fused with GST, and used them in ELISAs in order to overcome the cross-reactivity seen with B. caballi. One of the deletion clones, a clone with a deletion of the Be82 gene from positions 236 to 381 (Be82/236-381), specifically and sensitively detected B. equi-infected horse sera without cross-reactivity with B. caballi-infected horse sera. Assays with clones from which other gene products were deleted showed decreased sensitivities or remained nonspecific for the detection of B. equi-infected horse sera. These results suggest that the Be82/236-381 gene product is a novel antigen for the diagnosis of B. equi infection in horses.

机译：Be82基因产物与谷胱甘肽 S -转移酶（GST / Be82）融合在酶联免疫吸附测定（ELISA）中对诊断 Babesia equi 感染的功效为以前报道过（H.Hirata等人，J.Clin.Microbiol.40：1470-1474，2002）。然而，尽管 B的检出率很高，但具有GST / Be82抗原的ELISA却与 Babesia caballi 感染的马血清交叉反应。等。这些结果表明，GST / Be82具有与 B相同的抗原。 caballi 或抗原性类似于 B。 caballi 。在本研究中，我们构建了一系列五个Be82基因产物中缺失的克隆，每个克隆均与GST融合，并在ELISA中使用它们，以克服与 B所见的交叉反应性。 caballi 。缺失克隆之一是从位点236至381（Be82 / 236-381）具有Be82基因缺失的克隆，其被特异性地和灵敏地检测到了 B。被马感染的马血清，与 B无交叉反应。被Caballi 感染的马血清。用缺失了其他基因产物的克隆进行的测定显示灵敏度降低或对于检测 B仍非特异性。被马感染的马血清。这些结果表明，Be82 / 236-381基因产物是用于诊断 B的新型抗原。马匹感染马匹。 展开▼

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《Journal of Clinical Microbiology》 |2003年第2期|共5页

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Haruyuki Hirata; Xuenan Xuan; Naoaki Yokoyama; Yoshifumi Nishikawa; Kozo Fujisaki; Naoyoshi Suzuki;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Monoclonal Antibody against Babesia equi: Characterization and Potential Application of Antigen for Serodiagnosis [J] . Abgaandorjiin Avarzed, Ikuo Igarashi, Daniel T. De Waal, Journal of Clinical Microbiology . 1998,第7期

机译：马鞭虫病单克隆抗体：血清学诊断的抗原的表征和潜在应用。

2. Merozoite proteins from Babesia sp. BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA. [J] . Guan GQ, Chauvin A, Rogniaux H, Parasitology . 2010,第6期

机译：来自Babesia sp。的裂殖子蛋白。 BQ1（林丹）作为通过ELISA进行血清学诊断的潜在抗原。

3. Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis [J] . Goo YK, Jia H, Aboge GO, Parasitology International . 2009,第1期

机译：巴贝斯球藻12-kDa蛋白作为血清学诊断的潜在抗原的表征

4. The Babesia divergens Bd37 erythrocyte binding protein: structural analysis of a protective antigen [C] . Murciano B, Bres V, Auguin D, International Congress of Parasitology . 2010

机译：Babesia Divergens BD37红细胞结合蛋白：保护性抗原的结构分析

5. Molecular cloning, characterization, and immunogenicity of a species-specific Babesia microti antigen. [D] . Tetzlaff, Christine Lynn. 1988

机译：物种特异性巴贝斯微虫抗原的分子克隆，表征和免疫原性。

6. Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis [O] . Haruyuki Hirata, Xuenan Xuan, Naoaki Yokoyama, 2003

机译：马鞭毛虫巴氏杆菌P82蛋白特异抗原区的鉴定及其在血清学诊断中的潜在应用

7. Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis [O] . Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, 2003

机译：马鞭毛虫巴氏杆菌P82蛋白特异抗原区的鉴定及其在血清学诊断中的潜在应用

8. In vitro Antigen-Specific Antibody Response to the Species-Specific Surface Protein Antigens of Typhus Group Rickettsiae by Human Peripheral Blood Mononuclear Cells. Generation of an Antigen-Dependent Suppressor T Cell [R] . Misiti, J., Dasch, G. A. 1985

机译：体外抗原特异性抗体对人外周血单个核细胞对斑疹伤寒组立克次体的物种特异性表面蛋白抗原的反应。产生抗原依赖性抑制T细胞

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5. 肺炎支原体ORF6区蛋白重组抗原制备及在血清学诊断中的初步应用 [J] . 王惠榕 ,高岚美 ,萧剑雄 . 中国人兽共患病学报 . 2011,第007期

6. 基于抗原抗体反应的牛源A型多杀性巴氏杆菌保护性抗原的筛选鉴定 [C] . 李春明 ,任静静 ,杜慧慧 . 第七届全国牛病防制及产业发展大会 . -1

7. 基于抗原抗体反应持牛源A型多杀性巴氏杆菌保护性抗原的筛选及鉴定 [A] . 李春明 . 2015

1. 硒与猪多杀性巴氏杆菌抗原基因plpP联合在增强猪巴氏杆菌疫苗免疫保护中的应用 [P] . 中国专利： CN105543247B . 2017.08.25

2. 硒与猪多杀性巴氏杆菌抗原基因plpP联合在增强猪巴氏杆菌疫苗免疫保护中的应用 [P] . 中国专利： CN105543247A . 2016-05-04

3. equine immunization vaccine against babesiosis, process for isolating babesia equi merozoite apical complex proteins, proteins, use of proteins and method of immunization of equine babesiosis [P] . 外国专利： BRPI0600687A . 2007-11-20

机译：马鞭毛病的马免疫疫苗，分离巴贝斯虫的裂殖子根尖复合蛋白的方法，蛋白，蛋白的用途和马鞭毛病的免疫方法

4. isolated antigen binding region that is specific for cd38, isolated antibody or a functional fragment thereof that is specific for cd38, isolated antibody or a functional fragment thereof, isolated immunoglobulin, heavy chain variable of an isolated antigen binding region, variable light chain from an isolated antigen binding region, isolated nucleic acid sequence, nucleic acid sequence encoding a variable heavy chain from an isolated antigen binding region, nucleic acid sequence, vector, isolated cell, pharmaceutical composition, use of the pharmaceutical composition, method of inducing specific death of cd38-expressing tumor cells, method of detecting specific death of cd38-expressing tumor cells, method of detecting the presence of cd38 in a minipore source tissue or cell , cd38 detection method in a cd3-expressing erythrocyte 8 and diagnostic composition [P] . 外国专利： BRPI0618399A2 . 2011-08-30

机译：对cd38有特异性的分离的抗原结合区，对cd38有特异性的分离的抗体或其功能片段，分离的抗体或其功能片段，分离的免疫球蛋白，分离的抗原结合区的重链可变区，分离的可变轻链抗原结合区，分离的核酸序列，编码来自分离的抗原结合区的可变重链的核酸序列，核酸序列，载体，分离的细胞，药物组合物，药物组合物的用途，诱导cd38-特异性死亡的方法表达肿瘤细胞的方法，检测表达cd38的肿瘤细胞的特异性死亡的方法，检测小孔源组织或细胞中cd38的存在的方法，表达cd3的红细胞8的cd38检测方法和诊断组合物

5. Merozoite proteins for use in detection of Babesia equi in horses using immunological techniques [P] . 外国专利： US5643737A . 1997-07-01

机译：裂殖子蛋白，用于通过免疫学技术检测马匹马再性巴贝虫

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Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

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