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首页> 外文期刊>Journal of Clinical Microbiology >Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-β-Lactamase Gene blaIMP in a Hospital in Japan
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Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-β-Lactamase Gene blaIMP in a Hospital in Japan

机译：携带金属β-内酰胺酶基因blaIMP的恶臭假单胞菌菌株在日本一家医院中的存在

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To determine the persistence and spread of antibiotic-resistant strains in Gunma University Hospital, 83 Pseudomonas putida strains (each from a different patient) were isolated from January 1997 through December 2001. Of the 83 strains isolated, 27 were resistant to carbapenems. All 27 produced metallo-β-lactamase and were found to be PCR positive for the blaIMP gene. Most (22 strains) were primarily isolated from the wards (W7 [9 strains] and W4 [8 strains]). Another five blaIMP-positive P. putida strains from wards W7 and W4 were obtained by swabbing around the water pipes. A total of 32 blaIMP-positive P. putida strains were assessed by pulsed-field gel electrophoresis (PFGE) and testing of drug susceptibility to 10 chemotherapeutic agents. Both PFGE and MIC patterns revealed that there were long-term resident strains among inpatients and hospital environments. The blaIMP genes of 22 of 32 strains were all transferable to a recipient strain of Pseudomonas aeruginosa by conjugation or transformation and conferred resistance to carbapenems and cephems. The blaIMP plasmids were conjugally transmissible among P. aeruginosa strains and mediated resistance to amikacin as well as β-lactams. Ten of the 22 plasmids mediated additional resistance to gentamicin and tobramycin. Plasmids with identical DNA and drug resistance patterns were found in P. putida strains with identical PFGE patterns and with different PFGE patterns. We presumed that P. putida was one of the resident species in inpatients and especially in hospital environments, spreading drug resistance genes via plasmids among P. putida strains and supplying them to more pathogenically important species, such as P. aeruginosa.
机译：为了确定群马大学附属医院中抗生素耐药菌株的持久性和扩散性，从1997年1月至2001年12月分离了83株恶臭假单胞菌菌株（每株均来自另一名患者）。在分离出的83株菌株中，有27株对碳青霉烯类有抗性。全部27种产生的金属β-内酰胺酶均被发现对 bla IMP 基因呈PCR阳性。大多数（22株）主要从病房中分离出来（W7 [9株]和W4 [8株]）。另外五个 bla IMP 阳性 P。 W7和W4区的恶臭菌株是通过在水管周围擦拭而获得的。总共32个 bla IMP 阳性 P。通过脉冲场凝胶电泳（PFGE）评估了putida 菌株，并测试了其对10种化疗药物的敏感性。 PFGE和MIC模式均表明，住院患者和医院环境中存在长期的菌株。 32个菌株中的22个菌株的 bla IMP 基因都可以通过结合或转化转移到铜绿假单胞菌的受体菌株中，并赋予对碳青霉烯和头巾。 bla IMP 质粒可在 P之间共轭传递。铜绿假单胞菌菌株和对丁胺卡那霉素以及β-内酰胺的介导抗性。 22个质粒中的10个介导了对庆大霉素和妥布霉素的额外抗性。在 P中发现具有相同DNA和耐药模式的质粒。具有相同PFGE模式和不同PFGE模式的putida菌株。我们假设 P。 putida 是住院病人中的一种常驻物种，尤其是在医院环境中，它通过质粒在 P中传播耐药基因。 putida 菌株并将其提供给更具致病性的重要物种，例如 P。铜绿。

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