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首页> 外文期刊>Journal of Clinical Microbiology >Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence
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Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence

机译：Burkholderia pseudomallei克隆的非随机分布与地理位置和毒力的关系

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Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern Australia. Using multilocus sequence typing (MLST), we have compared (i) soil and invasive isolates from northeast Thailand and (ii) invasive isolates from Thailand and Australia. A total of 266 Thai B. pseudomallei isolates were characterized (83 soil and 183 invasive). These corresponded to 123 sequence types (STs), the most abundant being ST70 (n = 21), ST167 (n = 15), ST54 (n = 12), and ST58 (n = 11). Two clusters of related STs (clonal complexes) were identified; the larger clonal complex (CC48) did not conform to a simple pattern of radial expansion from an assumed ancestor, while a second (CC70) corresponded to a simple radial expansion from ST70. Despite the large number of STs, overall nucleotide diversity was low. Of the Thai isolates, those isolated from patients with melioidosis were overrepresented in the 10 largest clones (P < 0.0001). There was a significant difference in the classification index between environmental and disease isolates (P < 0.001), confirming that genotypes were not distributed randomly between the two samples. MLST profiles for 158 isolates from Australia (mainly disease associated) contained a number of STs (96) similar to that seen with the Thai invasive isolates, but no ST was found in both populations. There were also differences in diversity and allele frequency distribution between the two populations. This analysis reveals strong genetic differentiation on the basis of geographical isolation and a significant differentiation on the basis of virulence potential.
机译： Burkholderia pseudomallei 是居住在土壤中的腐生植物，是类拟li病的病原体，后者是威胁生命的人类感染。多数病例来自泰国东北部和澳大利亚北部。使用多基因座序列分型（MLST），我们比较了（i）泰国东北部的土壤和侵入性分离株以及（ii）泰国和澳大利亚的侵入性分离株。总共266个泰国 B。鉴定出假假麦芽孢杆菌分离株（83种土壤和183种侵入性）。这些对应于123个序列类型（ST），最丰富的是ST70（ n = 21），ST167（ n = 15），ST54（ n = 12）和ST58（ n = 11）。确定了两个相关的ST（克隆复合体）簇;较大的克隆复合体（CC48）不符合假定祖先的简单径向扩增模式，而第二克隆（CC70）对应于ST70的简单径向扩增。尽管有大量的ST，但总体核苷酸多样性较低。在泰国分离株中，从类li虫病患者中分离出的分离株在10个最大克隆中占过多（ P <0.0001）。环境和疾病分离株之间的分类指数存在显着差异（ P <0.001），这证实了两个样本之间的基因型不是随机分布的。来自澳大利亚（主要与疾病相关）的158个分离株的MLST图谱包含许多ST（96），与泰国侵入性分离株相似，但在两个人群中均未发现ST。两个种群之间的多样性和等位基因频率分布也存在差异。该分析揭示了基于地理隔离的强遗传分化和基于毒力潜力的显着分化。

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