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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the Bordetella pertussis and Bordetella parapertussis Isolates Circulating in Saint Petersburg between 1998 and 2000 with Russian Vaccine Strains
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Comparison of the Bordetella pertussis and Bordetella parapertussis Isolates Circulating in Saint Petersburg between 1998 and 2000 with Russian Vaccine Strains

机译：1998年至2000年在圣彼得堡流通的百日咳博德特氏菌和副百日咳博德特氏菌分离株与俄罗斯疫苗株的比较

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We analyzed the Bordetella pertussis and Bordetella parapertussis isolates circulating in Saint Petersburg that were collected between 1998 and 2000 and compared them with isolates collected 40 years ago and Russian vaccine strains. The analysis involved serotyping, pulsed-field gel electrophoresis of chromosomal DNA after digestion with XbaI and SpeI, and sequencing of the ptxS1 and prn genes, which encode the S1 subunit of the pertussis toxin and the major adhesin pertactin, respectively. The Russian isolates were classified in five of the six pulsed-field gel electrophoresis groups identified in other European countries. The B. pertussis isolates currently circulating in Saint Petersburg differed from the Russian whole-cell vaccine strains and the isolates collected in the prevaccine era. However, their repartition in the major pulsed-field gel electrophoresis groups was slightly different from that of isolates collected in countries that have had a high level of vaccine coverage for a long time, probably because the level of vaccine coverage in Saint Petersburg has increased only recently, after decreasing until the early 1990s. Most of the B. parapertussis isolates studied were similar to those circulating in France. However, some variants were observed, perhaps because B. parapertussis infections are more common in children in this area.
机译：我们分析了1998年至2000年在圣彼得堡流通的百日咳博德特氏菌和副百日咳博德特氏菌，并将它们与40年前的分离株和俄罗斯疫苗株进行了比较。分析涉及 Xba I和 Spe I消化后的染色体DNA的血清分型，脉冲场凝胶电泳，以及 ptxS1 和 em> prn 基因，分别编码百日咳毒素S1亚基和主要粘附素pertactin。在欧洲其他国家/地区中，俄罗斯分离株被分为六个脉冲场凝胶电泳组中的五个。 B。目前在圣彼得堡流通的百日咳菌与俄罗斯的全细胞疫苗株不同，疫苗分离株是在疫苗接种前收集的。但是，它们在主要脉冲场凝胶电泳组中的分配与疫苗覆盖率长期处于较高水平的国家/地区中分离到的分离株略有不同，可能是因为圣彼得堡的疫苗覆盖率仅在增加直到1990年代初期有所减少。大多数 B。副百日咳杆菌的分离株与法国流行的分离株相似。但是，观察到一些变体，可能是因为 B。百日咳杆菌感染在该地区的儿童中更为常见。

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