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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus
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Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus

机译：评价Binax NOW，BD Directigen和BD Directigen EZ检测用于检测呼吸道合胞病毒的方法

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The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staining (DFA) were compared with culture for detection of RSV in fresh specimens from both children and adults during the 2002-2003 respiratory virus season. The majority (95%) of specimens were nasal or nasopharyngeal washes or aspirates. A total of 47 (26%) were culture positive for RSV. The overall sensitivities of DFA (n = 149), NOW (n = 118), EZ (n = 88), and DRSV (n = 180) compared with culture (n = 180) were 93, 89, 59, and 77%, respectively. The specificities of DFA, NOW, EZ, and DRSV were 97, 100, 98, and 96%, respectively. However, when results were separated into those from children and those from adults, DFA was the only rapid test adequate for detection of RSV (sensitivity of 100% compared to 0, 0, and 25% for NOW, EZ, and DRSV, respectively) in adults. For children the sensitivities of DFA, NOW, EZ, and DRSV were 93, 94, 72, and 81%. The NOW assay was the most sensitive and specific and the easiest to perform of the kit tests for detecting RSV in children. None of these three rapid kit tests was sensitive for detecting RSV in specimens from adults. DFA remains the rapid method of choice for detecting RSV in the adult population.
机译：Binax NOW测定法（缅因州波特兰的Binax公司）和BD Directigen EZ测定法（马里兰州Sparks的Becton Dickinson and Company）是两种用于检测呼吸道合胞病毒（RSV）的新型快速免疫测定法，以及在2002-2003年呼吸道病毒季节，将BD Directigen RSV分析（DRSV）（Becton Dickinson and Company）和直接免疫荧光染色（DFA）与培养物相比较，以检测儿童和成人新鲜标本中的RSV。大部分标本（95％）为鼻腔或鼻咽洗液或吸出物。总计47（26％）对RSV培养呈阳性。 DFA（ n = 149），NOW（ n = 118），EZ（ n = 88）和DRSV（< em> n = 180）与文化（ n = 180）相比分别为93％，89、59和77％。 DFA，NOW，EZ和DRSV的特异性分别为97％，100％，98％和96％。但是，当将结果分为儿童和成人时，DFA是唯一足以检测RSV的快速测试（灵敏度为100％，而NOW，EZ和DRSV的灵敏度分别为0、0和25％）在成人中。对于儿童，DFA，NOW，EZ和DRSV的敏感性分别为93％，94％，72％和81％。 NOW分析是检测儿童RSV的最灵敏，特异性最高，最容易进行的试剂盒测试。这三种快速试剂盒测试对检测成人标本中的RSV都不敏感。 DFA仍然是检测成年人口RSV的快速选择方法。

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