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首页> 外文期刊>Journal of Clinical Microbiology >Selection of Strains for Quality Assessment of the Disk Induction Method for Detection of Inducible Clindamycin Resistance in Staphylococci: a CLSI Collaborative Study
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Selection of Strains for Quality Assessment of the Disk Induction Method for Detection of Inducible Clindamycin Resistance in Staphylococci: a CLSI Collaborative Study

机译：圆盘诱导法检测葡萄球菌中可诱导的克林霉素抗性的菌株的质量评估：CLSI合作研究

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摘要

A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four strains of Staphylococcus aureus were tested as unknowns on 10 different days in each laboratory using the recently recommended CLSI (formerly NCCLS) disk diffusion method and the inoculum purity control method. Strains contained either macrolide-lincosamide-streptogramin B (MLSB) resistance genes encoded by erm(A) or erm(C) or a macrolide resistance efflux pump encoded by msr(A). Based upon the results of this study, strain UT 32 (now designated ATCC strain BAA-977) containing erm(A) is recommended as the positive control organism for inducible clindamycin resistance. Strain UT 25 (now designated ATCC BAA-976), which harbors the efflux pump encoded by msr(A), is recommended as the negative control organism.
机译：进行了九个实验室的合作研究，以选择阳性和阴性质量评估对照菌株，以检测葡萄球菌中可诱导的克林霉素抗性。使用最新推荐的CLSI（以前称为NCCLS）圆盘扩散法和接种纯度控制方法，在每个实验室的10个不同天对4株金黄色葡萄球菌进行了未知测试。菌株含有 erm （A）或 erm （C）编码的大环内酯-林可酰胺-链霉菌素B（MLS B ）抗性基因或大环内酯类由 msr （A）编码的电阻外排泵。根据这项研究的结果，建议将含有 erm （A）的UT 32菌株（现称为ATCC菌株BAA-977）作为可诱导的克林霉素抗性的阳性对照。建议使用带有 msr （A）编码的外排泵的UT 25菌株（现称为ATCC BAA-976）作为阴性对照。

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