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首页> 外文期刊>Journal of Clinical Microbiology >Indirect Enzyme-Linked Immunosorbent Assay for Detection of Brucella melitensis-Specific Antibodies in Goat Milk
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Indirect Enzyme-Linked Immunosorbent Assay for Detection of Brucella melitensis-Specific Antibodies in Goat Milk

机译：间接酶联免疫吸附法检测羊奶中布鲁氏菌特异性抗体

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Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the U.S. goat population. The objective of this study was to develop a sensitive and specific indirect enzyme-linked immunosorbent assay (iELISA) for the detection of B. melitensis-specific antibodies in goat milk. Brucella salt-extractable protein extract was employed as an antigen, and a horseradish peroxidase-labeled polyclonal anti-goat antibody was used as an anti-species conjugate. Thirteen of 13 (100%) individual infected goat milk samples tested positive and 134 of 134 (100%) uninfected bulk milk samples tested negative by the developed iELISA. Three positive milk samples with high, medium, and low absorbance values were used to simulate one positive animal in an otherwise negative herd. By this estimation, one high-titer animal could be detected in a herd of >1,600 animals. Detection estimates for medium- and low-titer animals were one positive animal per herd of <200 and 50 animals, respectively. Based on this estimation, it is recommended that herds be sampled in groups of 50 animals or less for bulk milk testing. The iELISA developed for this study was found to be sensitive and specific and shows potential for use as a bulk milk test for the detection of B. melitensis-specific antibodies in goat milk.
机译：布鲁氏菌是绵羊和山羊布鲁氏菌病的病因，通常导致流产。 B的案例很少。在过去的25年中，美国已经发生了山羊melitensis 感染。但是，必须保持警惕，就像对牛乳业一样，以确保不会将布鲁氏菌病引入美国山羊种群。这项研究的目的是开发一种灵敏且特异性的间接酶联免疫吸附测定法（iELISA），用于检测 B。羊奶中的Melitensis 特异性抗体。将 Brucella 盐提取蛋白用作抗原，并将辣根过氧化物酶标记的多克隆抗山羊抗体用作抗物种结合物。通过开发的iELISA，在13个（100％）个体感染山羊奶样品中，有十三个测试为阳性，在134个（100％）未感染的散装牛奶样品中，有134个测试为阴性。使用三个具有高，中和低吸光度值的阳性乳样品来模拟否则为阴性的牛群中的一只阳性动物。通过这种估计，可以在超过1,600只动物的群中检测到一只高口径动物。中低效动物的检测估计数是每群<200和50只动物中的一只阳性动物。基于此估计，建议以50只或更少的动物为一组对牛群进行抽样，以进行批量牛奶测试。发现为这项研究开发的iELISA具有灵敏性和特异性，并显示出可能用作检测 B的大量牛奶测试的潜力。羊奶中的Melitensis 特异性抗体。

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