Identification of Two Distinct Hybridization Groups in the Genus Hafnia by 16S rRNA Gene Sequencing and Phenotypic Methods

J. Michael Janda; Sharon L. Abbott; Sue Bystrom; Will S. Probert

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Identification of Two Distinct Hybridization Groups in the Genus Hafnia by 16S rRNA Gene Sequencing and Phenotypic Methods

机译：通过16S rRNA基因测序和表型方法鉴定Hafnia属中两个不同的杂交基团

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A collection of 52 strains belonging to the Hafnia alvei complex were subjected to molecular (16S rRNA gene sequencing) and biochemical analysis. Based upon 16S rRNA gene sequencing results, two genetic groups were identified which correspond with previously recognized DNA hybridization group 1 (ATCC 13337T and ATCC 29926; n = 23) and DNA hybridization group 2 (ATCC 29927; n = 29). Of 46 biochemical tests used to characterize hafniae, 19 reactions (41%) yielded variable results. Of these 19 tests, 6 were determined to have discriminatory value in the separation of DNA groups 1 and 2, with malonate utilization found to be the most differential test. Test results of malonate utilization alone correctly assigned 90% of Hafnia isolates to their correct DNA group.

机译：收集了52个属于'Hafnia alvei 复合体的菌株，并进行了分子（16S rRNA基因测序）和生化分析。根据16S rRNA基因测序结果，确定了两个基因组，分别对应于先前公认的DNA杂交组1（ATCC 13337 T 和ATCC 29926; n = 23）和DNA杂交组2（ATCC 29927; n = 29）。在用于表征哈弗菌的46种生化测试中，有19种反应（41％）产生了可变结果。在这19项测试中，有6项被确定具有区分DNA组1和2的价值，丙二酸利用率是最有差异的测试。仅丙二酸利用率的测试结果就将90％的 Hafnia 菌株正确地分配到了其正确的DNA组。 展开▼

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《Journal of Clinical Microbiology》 |2005年第7期|共4页

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J. Michael Janda; Sharon L. Abbott; Sue Bystrom; Will S. Probert;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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6. Identification of Two Distinct Hybridization Groups in the Genus Hafnia by 16S rRNA Gene Sequencing and Phenotypic Methods [O] . J. Michael Janda, Sharon L. Abbott, Sue Bystrom, 2005

机译：通过16S rRNA基因测序和表型方法鉴定Hafnia属中两个不同的杂交基团

7. Identification of Two Distinct Hybridization Groups in the Genus Hafnia by 16S rRNA Gene Sequencing and Phenotypic Methods [O] . Janda, J. Michael, Abbott, Sharon L., Bystrom, Sue, 2005

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Identification of Two Distinct Hybridization Groups in the Genus Hafnia by 16S rRNA Gene Sequencing and Phenotypic Methods

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