Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes

Yi Chen; Wei Zhang; Stephen J. Knabel

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Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes

机译：多毒力基因座序列分型识别单核苷酸多态性，以区别于李斯特菌的流行性克隆和暴发菌株。

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A recently developed multi-virulence-locus sequence typing (MVLST) method showed improved discriminatory power for subtyping genetically diverse Listeria monocytogenes isolates and identified epidemic clone II isolates associated with two recent U.S. multistate listeriosis outbreaks. To evaluate the ability of MVLST to distinguish other epidemic clones and outbreak strains of L. monocytogenes, 58 outbreak-related isolates from 14 outbreaks and 49 unrelated isolates were analyzed. Results showed that MVLST provided very high discriminatory power (0.99), epidemiological concordance (1.0), stability, and typeability. MVLST accurately identified three previously known epidemic clones (epidemic clones I, II, and III) and redefined another epidemic clone (epidemic clone IV) in serotype 4b of L. monocytogenes. A set of 28 single nucleotide polymorphisms (SNPs) differentiated all epidemiologically unrelated isolates. A subset of 16 SNPs differentiated all epidemic clones and outbreak strains. Phylogenetic analysis showed congruence between MVLST clusters, serotypes, and previously defined genetic lineages of L. monocytogenes. SNPs in virulence genes appear to be excellent molecular markers for the epidemiological investigation of epidemics and outbreaks caused by L. monocytogenes.

机译：最近开发的多毒力基因座序列分型（MVLST）方法显示出对亚型遗传多样的单核细胞增生李斯特氏菌分离株亚型的鉴别能力增强，并鉴定了与最近两次美国多州李斯特菌病暴发有关的流行性克隆II分离株。为了评估MVLST区分其他流行性克隆和 L暴发株的能力。对单核细胞增生病进行了分析，分析了14例暴发中58例与暴发相关的分离株和49例不相关的分离株。结果表明，MVLST具有很高的判别力（0.99），流行病学一致性（1.0），稳定性和可打字性。 MVLST准确地鉴定了三个先前已知的流行克隆（流行性克隆I，II和III），并在L型血清型4b中重新定义了另一个流行性克隆（流行性克隆IV）。单核细胞增生。一组28个单核苷酸多态性（SNP）区分了所有与流行病学无关的分离株。 16个SNP的子集区分了所有流行克隆和暴发菌株。系统发育分析表明，MVLST簇，血清型和以前确定的 L遗传谱系之间具有一致性。单核细胞增生。毒力基因中的SNP似乎是 L引起的流行病和暴发流行病学调查的出色分子标记。单核细胞增生。 展开▼

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《Journal of Clinical Microbiology》 |2007年第3期|共12页

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Yi Chen; Wei Zhang; Stephen J. Knabel;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Prophages in Listeria monocytogenes Contain Single-Nucleotide Polymorphisms That Differentiate Outbreak Clones within Epidemic Clones [J] . Yi Chen, Stephen J. Knabel Journal of Clinical Microbiology . 2008,第4期

机译：单核细胞增生李斯特菌中的噬菌体包含单核苷酸多态性，可区分流行性克隆内的暴发克隆

2. Novel Multiplex Single Nucleotide Polymorphism-Based Method for Identifying Epidemic Clones of Listeria monocytogenes [J] . Sara Lomonaco, Stephen J. Knabel, Alessandra Dalmasso, Applied and Environmental Microbiology . 2011,第17期

机译：基于多重多重单核苷酸多态性的单核细胞增生性李斯特菌的克隆鉴定方法

3. Novel Multiplex Single Nucleotide Polymorphism-Based Method for Identifying Epidemic Clones of Listeria monocytogenes [J] . Sara Lomonaco, Stephen J. Knabel, Alessandra Dalmasso, Applied Microbiology . 2011,第17期

机译：基于多重多重单核苷酸多态性的单核细胞增生性李斯特菌的克隆鉴定方法

4. Using of statistical properties of GB-speckles coding the nucleotide sequences of the 'housekeeping'genes of Listeria monocytogenes for characterization of differences between ST7 and ST106 of the clonal complex CC7 [C] . Onega Ulianova, Sergey Zaytsev, Alexander Ulyanov, Saratov Fall Meeting;SPIE Conference on Optical and Nano-Technologies for Biology and Medicine;International Symposium on Optics and Biophotonics . 2020

机译：使用GB斑点的统计特性编码李斯特省单核细胞蛋白单核细胞增生的“内核心腺籽的核苷酸序列”，表征克隆复合CC7的ST7和ST106之间的差异

5. Characterization of Listeria monocytogenes Plasmids that were Newly Identified in Whole-genome Sequences of Listeriosis Outbreak Isolates. [D] . Sagert, Jason. 2014

机译：李斯特菌李斯特菌的表征新近鉴定为李斯特菌病暴发分离株全基因组序列的质粒。

6. Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes [O] . Yi Chen, Wei Zhang, Stephen J. Knabel 2012

机译：多毒力基因座序列分型识别单核苷酸多态性以区别于李斯特菌的流行性克隆和暴发菌株。

7. Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes▿ [O] . Chen, Yi, Zhang, Wei, Knabel, Stephen J. 2007

机译：多毒力基因座序列分型可鉴定单核苷酸多态性，该多态性可区分单核细胞增生性李斯特菌的流行克隆和暴发株

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2. 食源性单核细胞增生李斯特菌毒力岛4携带株的分型、毒力及生物被膜形成能力的测定 [J] . 刘扬扬 ,康立超 ,马勋 . 中国预防兽医学报 . 2020,第006期

3. 105株携带耶尔森菌强毒力岛大肠杆菌的毒力基因型和部分菌株毒力因子序列研究 [J] . 吕殿红 ,魏文康 ,黄忠 . 中国预防兽医学报 . 2012,第001期

4. 单增李斯特菌三重实时荧光PCR检测的建立及其毒力基因在分离菌株中分布 [J] . 刘二龙 ,袁慕云 ,吕英姿 . 中国人兽共患病学报 . 2016,第005期

5. 一多重耐药单增李斯特菌株的生长特性及毒力分析 [J] . 邵美丽 ,许岩 ,赵燕丽 . 微生物学杂志 . 2011,第002期

6. 应用流行性出血热单克隆抗体检测病人白细胞内出血热病毒抗原分型与肾损害关系的研究 [C] . 崔淑清 ,朱虹娟 ,李彦清 . 全国流行性出血热临床专题学术会议 . 1986

7. 不同毒力单增李斯特菌LIPI-1全序列分析及致病性差异研究 [A] . 伊娜娜 . 2012

1. 一种基于单核苷酸多态性的葡萄球菌种水平鉴定和菌株分型一体化方法 [P] . 中国专利： CN107164512B . 2021.03.09

2. 一种基于单核苷酸多态性的葡萄球菌种水平鉴定和菌株分型一体化方法 [P] . 中国专利： CN107164512A . 2017-09-15

3. Process for identifying translated expression proteins from a nucleotide sequence, purified recombinant protein, nucleotide sequence, screening processes for identifying expression clones in a library, for screening a library of nucleotide sequences, for identifying antigenic gene sequences for a sample of nucleotide sequences and to identify a therapeutic antigenic gene sequence, recombinant vector, host cell, amino acid sequence, isolated and purified polypeptide, antibody, and process for treating an infection. [P] . 外国专利： BR9915066A . 2002-01-15

机译：从核苷酸序列，纯化的重组蛋白，核苷酸序列鉴定翻译的表达蛋白的方法，在文库中鉴定表达克隆，筛选核苷酸序列的文库，鉴定核苷酸序列样品的抗原基因序列并鉴定的筛选方法治疗性抗原基因序列，重组载体，宿主细胞，氨基酸序列，分离和纯化的多肽，抗体以及治疗感染的方法。

4. Nucleic acid sequence, amino acid sequence, replicative cloning vector, isolated host cell expression vector, methods for testing a substance as a therapeutic agent for bone modulation in a host to identify a molecule and a candidate protein involved in bone modulation, to test for hbm activity, for pharmaceutical development to treat bone development disorders, to treat a bone development disorder in an animal, to alter bone development in a host, to treat osteoporosis, to evaluate diagnosis for a genetic predisposition to a bone development disorder, to identify a genetic predisposition to bone development disorders, to express hbm protein in bone tissue, to amplify a nucleotide polymorphism, to identify a regulatory element of an hbm gene, and to modulate bone density and in one patient, diagnostic assay for bone development disorders, bacterial artificial chromosome, isolated nucleic acid segment, nucleic acid, and polypeptide. [P] . 外国专利： BR0017197A . 2003-01-14

机译：核酸序列，氨基酸序列，复制性克隆载体，分离的宿主细胞表达载体，在宿主中测试作为骨调节治疗剂的物质的方法以鉴定参与骨调节的分子和候选蛋白以测试hbm活性，用于药物开发以治疗骨骼发育异常，治疗动物骨骼发育异常，改变宿主的骨骼发育，治疗骨质疏松症，评估对骨骼发育异常的遗传易感性诊断，鉴定遗传易患骨发育障碍，在骨组织中表达hbm蛋白，扩增核苷酸多态性，鉴定hbm基因的调节元件并调节骨密度，并且在一名患者中进行骨发育障碍的诊断测定，细菌人工染色体，分离的核酸片段，核酸和多肽。

5. Detecting target nucleotide sequences, useful e.g. for genotyping and analysis of single nucleotide polymorphisms, by reaction with all oligonucleotides in a single reaction chamber [P] . 外国专利： DE102005029810A1 . 2006-12-28

机译：检测靶核苷酸序列，例如通过与单个反应室中的所有寡核苷酸反应进行基因分型和单核苷酸多态性分析

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Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes

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