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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Commercial Antisera for Salmonella Serotyping
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Evaluation of Commercial Antisera for Salmonella Serotyping

机译：沙门氏菌血清分型的商业抗血清评估

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We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (Tokyo, Japan) somatic and flagellar antisera (using a tube agglutination assay) were 94.0% and 99.2%, respectively, and the specificity was 100% for both sets of sera. Polyvalent O and O1 antiserum sensitivity and specificity were >90%, with the exception of polyvalent O1 antiserum, for which sensitivity was 88.9%. When Denka Seiken flagellar antisera were used in a slide agglutination assay, the sensitivity and accuracy dropped to 88.9% and the specificity fell to 91%. Overall, Denka Seiken commercial antisera performed very well and, together with the comprehensive range of factors available, offer laboratories quality reagents suitable for serotyping strains of salmonellae.
机译：我们使用327株肠沙门氏菌（Salmonella enterica ）菌株，将加利福尼亚州公共卫生部微生物疾病实验室的一组商业沙门氏菌（Salmonella ）体检和鞭毛血清型抗血清与内部制备的抗血清进行了比较。属于I，II，IIIa，IIIb和IV子组。 Denka Seiken（日本东京）的体细胞和鞭毛抗血清（使用试管凝集法）的敏感性分别为94.0％和99.2％，两组血清的特异性均为100％。多价O和O1抗血清的敏感性和特异性均> 90％，多价O1抗血清的敏感性为88.9％。当在载玻片凝集测定中使用Denka Seiken鞭毛抗血清时，灵敏度和准确度下降至88.9％，特异性下降至91％。总体而言，Denka Seiken商业抗血清的性能非常好，并与各种因素结合在一起，为实验室提供了适用于沙门氏菌血清分型菌株的优质试剂。

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