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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Molecular Typing Methods for Characterization of Staphylococcus epidermidis: Proposal for Clone Definition
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Comparison of Molecular Typing Methods for Characterization of Staphylococcus epidermidis: Proposal for Clone Definition

机译：表皮葡萄球菌表征的分子分型方法比较：克隆定义的建议。

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In the present study we give some direction on the selection of the most appropriate typing method(s) to be used for the characterization of Staphylococcus epidermidis, in view of the most recent findings on the evolution, population structure, and epidemiology of this species. In order to achieve this aim, quantitative assessment of the correlation of the results of three typing methods—pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal chromosomal cassette mec (SCCmec) typing, which target different regions of the chromosome that evolve at different rates—was performed. In order to evaluate the discriminatory ability and the strength and direction of the correlation of the different typing methods, Simpson's index of diversity (SID), the adjusted Rand coefficient (AR), and the Wallace coefficient (W) were calculated. PFGE was the most discriminatory method (SID = 99%), followed by MLST (SID = 90%) and SCCmec typing (SID = 75%). The values of AR and W (0.10 < AR < 0.30; 0.50 < W < 0.75) indicated that the partition of the same isolate collection by PFGE, MLST, and SCCmec typing provided results that had only a poor correlation with each other. However, the information provided by the combination of PFGE and SCCmec enabled the prediction of the results obtained by MLST at the level of the clonal complex with a high degree of precision (W > 0.90). We propose that clones of S. epidermidis be defined by the combination of the PFGE type followed by the SCCmec type, which provides reliable information on the short-term epidemiology and the ability to predict with consistency long-term clonal evolution.
机译：在本研究中，鉴于进化，种群结构的最新发现，我们为选择用于表征表皮葡萄球菌的最合适的分型方法提供了一些指导，以及该物种的流行病学。为了实现这一目标，定量评估三种分型方法的结果之间的相关性：脉冲场凝胶电泳（PFGE），多位点序列分型（MLST）和葡萄球菌染色体盒 mec （SCC）进行了 mec ）分型，这些分型针对染色体上以不同速率进化的不同区域。为了评估辨别能力以及不同打字方法的相关性的强度和方向，使用辛普森多样性指数（SID），调整后的兰德系数（AR）和华莱士系数（ W ）进行了计算。 PFGE是最有区别的方法（SID = 99％），其次是MLST（SID = 90％）和SCC mec 分型（SID = 75％）。 AR和 W 的值（0.10 W <0.75）表明同一分离株集合由PFGE，MLST和SCC < em> mec 键入提供的结果彼此之间的关联性很差。但是，由PFGE和SCC mec 组合提供的信息可以预测MLST在克隆复合体水平上获得的结果的精确度（ W S的克隆。表皮动物由PFGE类型与SCC mec 类型的组合定义，它提供了有关短期流行病学的可靠信息，并具有预测长期克隆进化的能力。

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