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首页> 外文期刊>Journal of Clinical Microbiology >Production of species-specific murine monoclonal antibodies against Cryptococcus neoformans which recognize a noncapsular exoantigen.
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Production of species-specific murine monoclonal antibodies against Cryptococcus neoformans which recognize a noncapsular exoantigen.

机译：抗新隐球菌的物种特异性鼠类单克隆抗体的产生，该抗体识别非荚膜外抗原。

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Three monoclonal antibodies (MAbs), designated 7C5, 7C9, and 5G8, against a cytoplasmic antigen of Cryptococcus neoformans were produced. MAbs 7C5 and 7C9 recognize culture filtrate antigen (exoantigen) of both encapsulated and nonencapsulated isolates of this pathogen, which suggests that they do not recognize capsular polysaccharide material. This is supported by immunofluorescence data which show reactivity of all 3 MAbs to cytoplasm and cell membranes only. MAb 7C9 also recognized C. neoformans var. gattii antigens but no other fungal pathogens tested in an enzyme-linked immunosorbent assay, while 7C5 and 5G8 recognized antigens of the cross-reactive pathogen Trichosporon beigelii but did not recognize either C. neoformans var. gattii isolates or any other fungal antigens. By Western blot (immunoblot), 7C9 detected antigen at 110 to 120, 65 to 70, 45 to 50, and 36 to 38 kDa; in addition to the latter band, the other two MAbs recognized a band at approximately 30 kDa. All three MAbs were of the immunoglobulin G1 subclass. The two MAbs which are capable of reacting with noncapsular culture supernatant antigen have possible uses in serodiagnosis, particularly in AIDS patients infected with C. neoformans, since in this group the present latex agglutination test has some limitations.
机译：产生了三种针对新型隐球菌胞质抗原的单克隆抗体（MAb），分别命名为7C5、7C9和5G8。单克隆抗体7C5和7C9识别该病原体的封装和非封装分离物的培养滤液抗原（外抗原），这表明它们不识别荚膜多糖物质。免疫荧光数据支持了这一点，该数据仅显示了所有3种单克隆抗体对细胞质和细胞膜的反应性。单抗7C9也识别新孢梭菌变种。加蒂抗原，但没有其他真菌病原体在酶联免疫吸附试验中进行测试，而7C5和5G8识别交叉反应病原体Trichosporon米色丽的抗原，但不识别新孢梭菌。加蒂分离物或任何其他真菌抗原。通过Western印迹（免疫印迹），7C9检测到110至120、65至70、45至50和36至38kDa的抗原。除了后一个波段，其他两个单抗都识别出大约30 kDa的波段。所有三个MAb均为免疫球蛋白G1亚类。能够与非荚膜培养物上清液抗原反应的两种单克隆抗体在血清学诊断中可能具有用途，尤其是在感染新孢梭菌的艾滋病患者中，因为目前的乳胶凝集试验在这一组中有一定的局限性。

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