...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms.
【24h】

Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms.

机译：通过核糖分型，任意引物PCR和定位限制性位点多态性，从血清型硬乔中分离钩端螺旋体。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR (AP-PCR) fingerprinting, and the study of mapped restriction site polymorphisms (MRSPs) in rrs and rrl genes. After restriction of chromosomal DNA with BglII, EcoRI, or HindIII, each genotype was individualized with a distinct ribotype. The fingerprints produced by AP-PCR with seven primers clearly separated the two groups; primers KF and RSP produced species-specific products which assigned hardjoprajitno and hardjobovis isolates to the species L. interrogans sensu stricto and L. borgpetersenii, respectively. Furthermore, AP-PCR fingerprints gave evidence of a considerable genomic heterogeneity at the strain level among the hardjobovis group. Conversely, the hardjoprajitno group was homogeneous. MRSP profiles in ribosomal genes indicated that hardjoprajitno and hardjobovis isolates belonged to L. interrogans MRSP group B and L. borgpetersenii group C, respectively. AP-PCR and determination of MRSPs in ribosomal genes proved to be quick and reliable methods for typing Leptospira strains and for studying intraspecific population structures.
机译：Hardjoprajitno和hardjobovis基因型的钩端螺旋体血清型Hardjo分离株的特征是核糖分型，任意引物PCR（AP-PCR）指纹图谱以及rrs和rrl基因的限制性位点多态性（MRSP）定位研究。用BglII，EcoRI或HindIII限制染色体DNA后，每种基因型都用独特的核糖型进行个体化。 AP-PCR用七种引物产生的指纹清楚地将两组分开。引物KF和RSP产生了特定于物种的产品，分别将hardjoprajitno和hardjobovis分离物分配给interrogans sensu stricto和L. borgpetersenii物种。此外，AP-PCR指纹图谱表明在Hardjobovis组中菌株水平上存在相当大的基因组异质性。相反，hardjoprajitno组是同质的。核糖体基因中的MRSP谱图表明，hardjoprajitno和hardjobovis分离株分别属于询问人乳杆菌MRSP B组和Borgpetersenii C组。 AP-PCR和核糖体基因中MRSP的测定被证明是键入钩端螺旋体菌株和研究种内种群结构的快速可靠的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号