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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for hematological malignancies.
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Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for hematological malignancies.

机译：使用支气管肺泡灌洗液进行抗原检测和PCR测定法在接受血液系统恶性肿瘤治疗的患者中诊断侵袭性肺曲霉病的比较。

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The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid samples from 19 patients who were treated for hematological malignancies and who were suspected of having invasive pulmonary aspergillosis (IPA). All patients had fever and pulmonary infiltrates on the chest roentgenogram on the day that the BAL fluid was obtained. The ELISA results were compared with the results of culture and Aspergillus genus-specific PCR analysis of BAL fluid samples. ELISA was also performed with serum samples. Aspergillus species were detected by PCR or ELISA with BAL fluid samples from five of seven patients who had radiological evidence of IPA. Serum ELISA results were positive for all patients with ELISA-positive BAL fluid, and for four patients the serum ELISA was positive before the BAL fluid was obtained. PCR and ELISA were positive for 2 and 1 of 10 BAL fluid samples, respectively, obtained from patients without radiological evidence of IPA, and 5 and 2 of 35 BAL fluid samples, respectively, obtained from nonneutropenic patients. This preliminary investigation suggests that GM may be detected by ELISA in BAL fluid samples from patients at risk of IPA, but that monitoring of serum GM levels may allow for the earlier diagnosis of IPA. However, further evaluation in prospective studies is required.
机译：在19例接受过血液系统恶性肿瘤治疗并被怀疑患有浸润性肺曲霉病（IPA）的患者的支气管肺泡灌洗液（BAL）中，对夹心酶联免疫吸附测定（ELISA）检测半乳曲霉半乳甘露聚糖（GM）的性能进行了评估。 ）。在获得BAL液的当天，所有患者的胸部X线胸片上都有发烧和肺部浸润。将ELISA结果与BAL液体样品的培养结果和曲霉属特异性PCR分析结果进行比较。 ELISA也用血清样品进行。通过PCR或ELISA方法，对7例具有IPA放射学证据的患者中的5例BAL液体样品检测曲霉菌。所有具有ELISA阳性BAL液的患者的血清ELISA结果均为阳性，而四名患者在获得BAL液之前血清ELISA呈阳性。从没有IPA放射学证据的患者中分别获得10个BAL液体样本中的2个和1个的PCR和ELISA阳性，从非中性粒细胞减少的患者分别从35个BAL液体样本中的5个和2个阳性。这项初步研究表明，可以通过ELISA在患有IPA风险的患者的BAL液样本中检测到GM，但是监测血清GM水平可以使IPA的早期诊断成为可能。但是，需要在前瞻性研究中进行进一步评估。

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