...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Identification of Helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein.
【24h】

Identification of Helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein.

机译：基于物种特异性单克隆抗体与表面暴露蛋白反应的免疫斑点印迹法鉴定幽门螺杆菌。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Monoclonal antibodies (MAbs) against membrane preparations of Helicobacter pylori were produced. One MAb was found to be specific for H. pylori, because it did not react with a number of other bacterial species, including Helicobacter felis and Campylobacter jejuni. This MAb reacted with a 30-kDa protein found in outer membrane preparations of H. pylori. The protein was also detected on the cell surface on intact bacteria when analyzed by immunoelectron microscopy. To facilitate the identification of H. pylori isolates after culturing of biopsies, an immunodot blot assay based on the reaction of this MAb was developed. This assay was found to be highly specific for H. pylori. Sixty-six clinical isolates typed as H. pylori by conventional biochemical tests were found to be positive, whereas no other bacterial species tested gave a positive result. By this method, reliable and rapid identification of H. pylori could be accomplished.
机译：产生了针对幽门螺杆菌膜制剂的单克隆抗体（MAb）。发现一种单克隆抗体对幽门螺杆菌具有特异性，因为它不与许多其他细菌种类反应，包括猫幽门螺杆菌和空肠弯曲菌。该MAb与幽门螺杆菌外膜制剂中发现的30kDa蛋白反应。通过免疫电子显微镜分析时，还可以在完整细菌的细胞表面上检测到该蛋白。为了便于在活检组织培养后鉴定幽门螺杆菌分离株，开发了基于该MAb反应的免疫斑点印迹测定法。发现该测定法对幽门螺杆菌具有高度特异性。通过常规的生化测试，发现有66种临床分离株被归类为幽门螺杆菌，呈阳性，而所测试的其他细菌均未得到阳性结果。通过这种方法，可以可靠，快速地鉴定幽门螺杆菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号