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首页> 外文期刊>Journal of Clinical Microbiology >Differentiation between Candida dubliniensis andCandida albicans by Fatty Acid Methyl Ester Analysis Using Gas-Liquid Chromatography
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Differentiation between Candida dubliniensis andCandida albicans by Fatty Acid Methyl Ester Analysis Using Gas-Liquid Chromatography

机译：气相色谱法通过脂肪酸甲酯分析区分杜氏念珠菌和白色念珠菌

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Candida dubliniensis is often found in mixed culture with C. albicans, but its recognition is hampered as the color of its colonies in primary culture on CHROMagar Candida varies. Furthermore, definite identification of C. dubliniensis is difficult to achieve, time-consuming, and expensive. Therefore, a method to discriminate between these two closely related yeast species by fatty acid methyl ester (FAME) analysis using gas-liquid chromatography (Sherlock Microbial Identification System [MIS]; MIDI, Inc., Newark, Del.) was developed. Although the chromatograms of these two species revealed no obvious differences when applying FAME analysis, a new library (CADLIB) was successfully created using Sherlock Library Generation Software (MIDI). The amount and frequency of FAME was analyzed using library training files (n = 10 for each species), preferentially those comprising reference strains. For testing the performance of the CADLIB, clinical isolates genetically assigned to the respective species (C. albicans, n = 32; C. dubliniensis, n = 28) were chromatographically analyzed. For each isolate tested, MIS computed a similarity index (SI) indicating a hierarchy of possible strain fits. When using the newly created library CADLIB, the SIs for C. albicans andC. dubliniensis ranged from 0.11 to 0.96 and 0.53 to 0.93 (for all but one), respectively. Only three isolates of C. albicans (9.4%) were misidentified as C. dubliniensis, whereas all isolates of C. dubliniensiswere correctly identified. Resulting differentiation accuracy was 90.6% for C. albicans and 100% for C. dubliniensis. Cluster analysis and principal component analysis of the resulting FAME profiles showed two clearly distinguishable clusters matching up with two assigned species for the strains tested. Thus, the created library proved to be well suited to discriminate between these two species.
机译： Candida dubliniensis 通常与C混合培养。白色念珠菌，但由于其在CHROMagar念珠菌原代培养中的菌落颜色不同，因此其识别受到阻碍。此外，可以确定 C。 dubliniensis 难以实现，耗时且昂贵。因此，开发了一种使用气液色谱法（Sherlock Microbial Identification System [MIS]; MIDI，Inc.，Newark，DEL）通过脂肪酸甲酯（FAME）分析来区分这两个紧密相关的酵母种类的方法。尽管在应用FAME分析时，这两种物质的色谱图显示没有明显差异，但是使用Sherlock库生成软件（MIDI）成功创建了一个新库（CADLIB）。使用库训练文件（每个物种的 n = 10）分析FAME的数量和频率，优先选择那些包含参考菌株的文件。为了测试CADLIB的性能，我们对这些物种进行了基因分离的临床分离株（ C。albicans ， n = 32; C。dubliniensis ， n = 28）进行色谱分析。对于每个测试的分离物，MIS计算出一个相似性指数（SI），该指数指示可能的应变拟合的层次。使用新创建的库CADLIB时， C的SI。白色念珠和 C。 dubliniensis 的范围分别为0.11至0.96和0.53至0.93（除一个以外）。只有三个 C分离株。白色念珠菌（）（9.4％）被误认为是 C。 dubliniensis ，而所有 C的分离株。正确鉴定了dubliniensis 。对于 C，所得的区分精度为90.6％。白色念珠和 C的100％。 dubliniensis 。所得FAME谱的聚类分析和主成分分析显示，两个明显可区分的聚类与测试菌株的两个指定物种相匹配。因此，事实证明创建的库非常适合区分这两个物种。

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