...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Diagnostic value of anti-Candida enolase antibodies.
【24h】

Diagnostic value of anti-Candida enolase antibodies.

机译：抗念珠菌烯醇酶抗体的诊断价值。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An immunodominant antigen with enolase enzyme activity was purified and used for the development of an assay to detect antibodies directed against this antigen in sera from patients with either invasive candidiasis or Candida colonization. The Au enzyme-linked immunosorbent assay established with the Candida enolase antigen was able to discriminate significantly between invasive candidiasis and colonization in both immunocompetent and immunodeficient groups of patients. The test had a sensitivity of 50% and a specificity of 86% in the immunocompetent patient group. In the immunodeficient patient group, a sensitivity of 53% and a specificity of 78% were established. Antibody levels determined by a counterimmunoelectrophoresis assay with the same set of sera resulted in a better sensitivity for sera from the immunocompetent patient group but a lower specificity, i.e., 80 and 29%, respectively. The counterimmunoelectrophoresis assay of sera from the immunodeficient patient group was not able to discriminate significantly between invasive candidiasis and colonization. With the use of more serum from each patient, the sensitivity of the antibody detection assays increased, while the specificity was maintained. The increase, however, was not statistically significant. Combining the results of the antibody assays with antigen titers obtained by the Cand-Tec assay did not improve the predictive value with respect to invasive candidiasis, as determined by multivariance regression analysis. Furthermore, it was demonstrated by performance of Western blots (immunoblots) that sera from patients as well as a rabbit antiserum cross-reacted with the Candida enolase and baker's yeast enolase enzyme. However, by tandem crossed immunoelectrophoresis it was demonstrated that the antibodies were directed toward different epitopes of the antigen.
机译：纯化具有烯醇酶活性的免疫优势抗原，并将其用于检测从患有侵袭性念珠菌病或念珠菌定植的患者血清中针对该抗原的抗体的检测方法的开发。用念珠菌烯醇酶抗原建立的Au酶联免疫吸附试验能够在免疫能力强和免疫缺陷的患者组中明显区分浸润性念珠菌病和定植。该测试在具有免疫能力的患者组中的敏感性为50％，特异性为86％。在免疫缺陷患者组中，建立了53％的敏感性和78％的特异性。用相同的血清通过反免疫电泳测定法测定的抗体水平导致对具有免疫能力的患者组的血清具有更好的敏感性，但特异性较低，即分别为80％和29％。来自免疫缺陷患者组的血清的抗免疫电泳分析不能明显区分浸润性念珠菌病和定植。随着每位患者使用更多血清，抗体检测测定的灵敏度得以提高，同时特异性得以保持。但是，该增加在统计上并不显着。通过多方差回归分析确定，将抗体测定的结果与通过Cand-Tec测定获得的抗原滴度相结合并不能提高对浸润性念珠菌病的预测价值。此外，通过蛋白质印迹（免疫印迹）的表现证明了来自患者的血清以及与念珠菌烯醇酶和面包酵母酵母烯醇酶交叉反应的兔抗血清。然而，通过串联交叉免疫电泳证明，抗体针对抗原的不同表位。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号