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首页> 外文期刊>Journal of Clinical Microbiology >Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital.
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Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital.

机译：通过脉冲场凝胶电泳的流行病学研究在一家老年医院爆发了产生广谱β-内酰胺酶的肺炎克雷伯菌。

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Twelve cases of infections caused by extended-spectrum beta-lactamase (ESBla)-producing Klebsiella pneumoniae were reported between August 1991 and March 1993 in the Geriatric Department of the Nimes University Hospital, where these bacterial had not been previously isolated. Restriction profiles of total genomic DNAs cleaved by XbaI and SpeI were compared by pulsed-field gel electrophoresis. The strains that were tested included the 12 isolates from K. pneumoniae-infected patients, strains recovered from rectal swabs of asymptomatic patients in the same ward, and strains isolated in other hospitals in N?mes at the same time. The restriction profiles of the 12 isolates and those recovered from asymptomatic patients in the same ward were very similar. Over a period of more than 1 year, extended-spectrum beta-lactamases were not detected in K. pneumoniae isolates with restriction patterns different from that of the epidemic strain. It seems, therefore, that there was no transfer of a plasmid or a gene coding for ESBla to strains of K. pneumoniae that were different from the epidemic strain. At the same time, ESBla-producing K. pneumoniae isolates exhibiting restriction endonuclease profiles very different from that of the epidemic strain were isolated from other hospitals in N?mes. None of these strains caused an outbreak. Pulsed-field gel electrophoresis, which allows precise characterization of strains beyond the species level, is a useful tool for studying the ESBla-producing K. pneumoniae strains involved in nosocomial outbreaks.
机译：1991年8月至1993年3月之间，尼姆斯大学医院的老年科报告了十二例由产生超广谱β-内酰胺酶（ESBla）的肺炎克雷伯菌引起的感染病例。通过脉冲场凝胶电泳比较了XbaI和SpeI切割的总基因组DNA的限制性图谱。测试的菌株包括来自肺炎克雷伯菌感染患者的12株分离株，从同一病房中无症状患者的直肠拭子中回收的菌株，以及同时在尼美地区其他医院分离的菌株。在同一病房中，从无症状患者中回收的12种分离株的限制谱非常相似。超过一年的时间里，在肺炎克雷伯氏菌分离株中，与流行株不同的限制模式未检测到广谱β-内酰胺酶。因此，似乎没有将编码ESBla的质粒或基因转移到不同于该流行株的肺炎克雷伯氏菌菌株中。同时，从恩梅斯州的其他医院中分离出了生产ESBla的肺炎克雷伯菌分离株，它们表现出与流行毒株截然不同的限制性核酸内切酶谱。这些菌株均未引起暴发。脉冲场凝胶电泳技术可以精确鉴定超出物种水平的菌株，是研究涉及医院暴发的产ESBla的肺炎克雷伯菌菌株的有用工具。

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