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首页> 外文期刊>Journal of Clinical Microbiology >Molecular subtyping of Salmonella enteritidis phage type 8 strains from the United States.
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Molecular subtyping of Salmonella enteritidis phage type 8 strains from the United States.

机译：来自美国的肠炎沙门氏菌噬菌体8型菌株的分子亚型分析。

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Salmonella enteritidis is now the most common serotype of the genus Salmonella reported in the United States. Bacteriophage typing has been helpful for subdividing S. enteritidis strains from different sources in the United States. Most S. enteritidis outbreaks reported were egg related, and the majority of them were caused by strains of phage type 8. To determine whether restriction fragment length polymorphism of the rRNA genes (ribotyping) and of the genomic DNAs from two lysogenic phages from S. enteritidis could be used to discriminate between S. enteritidis phage type 8 strains, we conducted Southern hybridization studies on 24 isolates from different outbreaks and six non-outbreak-associated strains using DNA probes for 16S and 23S rRNA genes and S. enteritidis typing phages 1 and 2 from the Ward typing system (L. R. Ward, J. D. H. de Sa, and B. Rowe, Epidemiol. Infect. 99:291-294, 1987). Of seven restriction endonucleases screened with the probe for rRNA genes, AccI provided the best discrimination between strains; six distinct patterns were observed. AccI ribosomal DNA patterns 1 to 6 were detected among 76.7, 3.3, 6.7, 3.3, 3.3, and 6.7% of isolates tested, respectively. Strains of AccI ribosomal DNA pattern 3 could be further subdivided into two additional patterns by using SmaI. Epidemiologically related strains had identical patterns. No discrimination between strains was achieved by probes for phages 1 and 2. No sequences homologous to the phage I probe were detected among phage type 8 strains, and all strains tested with six restriction enzymes had the same hybridization pattern with the phage 2 probe. These findings demonstrate that ribotyping with AccI and SmaI provides an additional means of discriminating between some phage type 8 strains; however, ribotyping and the phage 2 hybridization results from egg-related outbreak strains support previous findings that these strains are closely related.
机译：肠炎沙门氏菌现在是美国报道的沙门氏菌最常见的血清型。噬菌体分型有助于从美国不同来源细分肠炎链球菌。报告的大多数肠炎沙门氏菌暴发与卵有关，并且大多数是由8型噬菌体菌株引起的。为了确定rRNA基因（核糖体分型）和来自两个S噬菌体噬菌体的基因组DNA的限制性片段长度多态性。肠炎沙门氏菌可用于区分8型肠炎沙门氏菌噬菌体，我们使用16S和23S rRNA基因的DNA探针和肠炎沙门氏菌分型噬菌体，对来自不同暴发的24种分离株和6种非暴发相关菌株进行了Southern杂交研究。和2分别来自Ward分型系统（LR Ward，JDH de Sa和B. Rowe，Epidemiol。Infect。99：291-294，1987）。在用探针筛选的rRNA基因的七个限制性核酸内切酶中，AccI可以最好地区分菌株。观察到六个不同的模式。在76.7％，3.3％，6.7％，3.3％，3.3％和6.7％的分离株中分别检测到AccI核糖体DNA模式1-6。通过使用SmaI，AccI核糖体DNA模式3的菌株可进一步细分为两个其他模式。流行病学相关菌株具有相同的模式。用噬菌体1和2的探针不能区分菌株。在噬菌体8型菌株中没有检测到与噬菌体I探针同源的序列，并且用六种限制酶测试的所有菌株与噬菌体2探针具有相同的杂交模式。这些发现表明，使用AccI和SmaI进行核糖分型提供了另一种区分某些8型噬菌体菌株的方法。然而，鸡蛋相关暴发菌株的核糖分型和噬菌体2杂交结果支持了以前的发现，即这些菌株密切相关。

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