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首页> 外文期刊>Journal of Clinical Microbiology >Genospecies Identification and Characterization of Lyme Disease Spirochetes of Genospecies Borrelia burgdorferi Sensu Lato Isolated from Rodents in Taiwan
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Genospecies Identification and Characterization of Lyme Disease Spirochetes of Genospecies Borrelia burgdorferi Sensu Lato Isolated from Rodents in Taiwan

机译：台湾啮齿动物伯氏疏螺旋体的莱姆病螺旋体的种质鉴定和鉴定

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Lyme disease spirochetes of the genospecies Borrelia burgdorferi sensu lato were identified and characterized for the first time in Taiwan. Seven isolates, designated TWKM1 to TWKM7, were purified from the ear tissues of three species of rodents captured from seven localities of Taiwan. The immunological characteristics of these Taiwan isolates were compared with those of other genospecies of Lyme disease spirochetes by analyzing the protein profiles and reactivities with B. burgdorferi-specific monoclonal antibodies (MAbs). The genospecies of these Taiwan isolates were also identified by the similarities in their plasmid profiles and differential reactivities with genospecies-specific PCR primers. Although two distinct protein profiles were observed among the seven Taiwan isolates, the MAb reactivities against the outer surface proteins of B. burgdorferi of all of these isolates were consistent with those of B. burgdorferi sensu lato. The similarities of the plasmid profiles also confirmed the identities of these Taiwan isolates. PCR analysis indicated that all of these Taiwan isolates were genetically related to the genospecies B. burgdorferi sensu stricto. These results demonstrate the first identification of Lyme disease spirochetes in Taiwan and also highlight the increasing demand for defining the reservoirs and vector ticks of B. burgdorferi. A serosurvey for Lyme disease infection in the human population of Taiwan may also be required.
机译：在台湾首次鉴定并鉴定了伯氏疏螺旋体（Borrelia burgdorferi sensu lato）的莱姆病螺旋体。从台湾七个地区捕获的三种啮齿动物的耳组织中纯化了七个分离株，命名为TWKM1至TWKM7。通过分析 B的蛋白质谱和反应性，将这些台湾分离株的免疫学特征与莱姆病螺旋体的其他基因型进行了比较。 Burburgeri 特异性单克隆抗体（MAb）。这些台湾分离株的基因型还通过其质粒图谱的相似性以及与基因型特异性PCR引物的差异反应性来鉴定。尽管在七个台湾分离株中观察到两个不同的蛋白质谱，但单克隆抗体对 B的外表面蛋白具有反应性。所有这些分离株的burgdorferi 与 B的一致。博格多菲里。质粒图谱的相似性也证实了这些台湾分离株的身份。 PCR分析表明，所有这些台湾分离株都与B基因型有关。 burgdorferi sensustricto。这些结果证明了台湾首次鉴定出莱姆病螺旋体，也突显了对定义 B的水库和媒介vector的需求不断增加。 burgdorferi 。还可能需要对台湾人口进行莱姆病感染的血清调查。

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