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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of a Type-Common Human Recombinant Monoclonal Antibody to Herpes Simplex Virus with High Therapeutic Potential
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Characterization of a Type-Common Human Recombinant Monoclonal Antibody to Herpes Simplex Virus with High Therapeutic Potential

机译：具有高治疗潜力的单纯型疱疹病毒类型人重组单克隆抗体的表征

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摘要

We report the characterization of a type-common human recombinant monoclonal antibody previously isolated by antigen selection from a phage-displayed combinatorial antibody library established from a herpes simplex virus (HSV)-seropositive individual. Competition with well-characterized murine monoclonal antibodies and immunodetection of gD truncations revealed that this antibody recognizes the group Ib antigenic site of glycoprotein D, a highly conserved and protective type-common determinant. To our knowledge, this is the first human group Ib monoclonal antibody ever described. The antibody also displayed first-order neutralization kinetics and a high neutralization rate constant, was capable of completely inhibiting syncytium formation by a fusogenic strain of HSV type 1, and efficiently neutralized low-passage clinical isolates of both HSV serotypes. Taken together with our earlier observations of the in vivo antiviral activities of this human recombinant antibody in animal models of HSV infection, the present results support the high therapeutic potential of this antibody.
机译：我们报告了特征类型的人类重组单克隆抗体的特征，该特征先前是通过从噬菌体展示的组合抗体库（从单纯疱疹病毒（HSV）-血清反应阳性的个体）建立的抗原选择中分离出来的。与特征明确的鼠类单克隆抗体的竞争和gD截短的免疫检测表明，该抗体识别糖蛋白D的Ib类抗原位点，糖蛋白D是高度保守和保护性类型的常见决定簇。据我们所知，这是有史以来第一个人类Ib类单克隆抗体。该抗体还显示出一级中和动力学和高中和速率常数，能够完全抑制1型HSV融合菌株的合胞体形成，并有效中和了两种HSV血清型的低通道临床分离株。结合我们在HSV感染动物模型中对该人类重组抗体的体内抗病毒活性的较早观察结果，目前的结果支持了该抗体的高治疗潜力。

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