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首页> 外文期刊>Journal of Clinical Microbiology >Cultivable Oral Microbiota of Domestic Dogs
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Cultivable Oral Microbiota of Domestic Dogs

机译：可养家犬口腔微生物群

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Bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16S rRNA gene sequencing. Among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. Approximately half of the phylotypes were identified to the species level, and 28% of these were considered members of the indigenous oral microbiota of humans. The 16S rRNA gene sequences of the remaining 44 phylotypes were not represented in GenBank, and most of these phylotypes were tentatively identified as candidate new species. The genera most frequently isolated from saliva were Actinomyces (26%), Streptococcus (18%), and Granulicatella (17%). The genera most frequently isolated from plaque were Porphyromonas (20%), Actinomyces (12%), and Neisseria (10%). A comparison of the DNA sequences from this study with sequences of the human microbiota available in GenBank showed that, on average, canine and human microbiotas differed by almost 7% in the 16S rRNA gene. In conclusion, this study has shown that the cultivable oral microbiotas of dogs and humans show significant differences.
机译：从九只狗的牙菌斑和其他五只狗的唾液样本中分离细菌，然后通过比较16S rRNA基因测序进行鉴定。在339个分离物中，鉴定出属于37属的84个不同系统型。大约一半的系统型被鉴定为物种水平，其中28％被认为是人类本土口腔微生物群的成员。其余44种系统型的16S rRNA基因序列未在GenBank中显示，并且大多数这些系统型被初步鉴定为候选新物种。从唾液中最常分离的属是放线菌（26％），链球菌（18％）和肉芽菌（17％）。从噬菌斑中最常分离的属是卟啉单胞菌（20％），放线菌（12％）和奈瑟氏球菌（emisseria）（10％）。将这项研究的DNA序列与GenBank中可用的人类微生物群进行比较，结果表明，犬和人类微生物群的16S rRNA基因平均相差近7％。总而言之，这项研究表明，狗和人的口腔可培养菌群之间存在显着差异。

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