...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections
【24h】

Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections

机译：525个样品的分析，以确定PCR扩增和16S rRNA基因测序在诊断骨和关节感染中的有用性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 16S rRNA gene PCR in the diagnosis of bone and joint infections has not been systematically tested. Five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16S rRNA gene PCR detection of bacteria in parallel. The amplicons with mixed sequences were also cloned. When discordant results were observed, culture and PCR were performed once again. Bacteria were detected in 139 of 525 samples. Culture and 16S rRNA gene PCR yielded identical documentation in 475 samples. Discrepancies were linked to 13 false-positive culture results, 5 false-positive PCR results, 9 false-negative PCR results, 16 false-negative culture results, and 7 mixed infections. Cloning and sequencing of 16S rRNA gene amplicons in 6 of 8 patients with mixed infections identified 2 to 8 bacteria per sample. Rarely described human pathogens such as Alcaligenes faecalis, Comamonas terrigena, and 21 anaerobes were characterized. We also detected, by 16S rRNA gene PCR, four previously identified bacteria never reported in human infection, Alkanindiges illinoisensis, dehydroabietic acid-degrading bacterium DhA-73, unidentified Hailaer soda lake bacterium, and uncultured bacterium clone HuCa4. Seven organisms representing new potential species were also detected. PCR followed by cloning and sequencing may help to identify new pathogens involved in mixed bone infection.
机译：用于骨和关节感染诊断的16S rRNA基因PCR尚未得到系统测试。培养了525例患者的525个骨和关节样品，并进行了16S rRNA基因PCR细菌平行检测。还克隆了具有混合序列的扩增子。当观察到不一致的结果时，再次进行培养和PCR。在525个样本中的139个中检测到细菌。培养和16S rRNA基因PCR在475个样品中产生了相同的文献资料。差异与13个假阳性培养结果，5个假阳性PCR结果，9个假阴性PCR结果，16个假阴性培养结果和7种混合感染相关。在8例混合感染患者中，有6例的16S rRNA基因扩增子的克隆和测序确定每个样品2至8个细菌。很少描述人类病原体，如粪便产碱杆菌，地上Comomonas terrigena以及21种厌氧菌。我们还通过16S rRNA基因PCR检测到了四种先前鉴定的人类感染中从未报道过的细菌， Alkanindiges illinoisensis ，脱氢松香酸降解细菌DhA-73，未鉴定的Hailaer苏打湖细菌和未培养的细菌克隆HuCa4。 。还发现了代表新的潜在物种的七个生物。 PCR，然后进行克隆和测序可能有助于鉴定参与混合骨感染的新病原体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号