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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of Clostridium difficile Isolates from Nosocomial Outbreaks at Three Hospitals in Diverse Areas of Japan
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Analysis of Clostridium difficile Isolates from Nosocomial Outbreaks at Three Hospitals in Diverse Areas of Japan

机译：日本不同地区三家医院医院爆发的艰难梭菌分离株的分析

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Clostridium difficile isolates recovered from patients with C. difficile-associated diarrhea (CDAD) at three hospitals located in diverse areas of Japan were analyzed by three typing systems, PCR ribotyping, pulsed-field gel electrophoresis (PFGE), and Western immunoblotting. At the three hospitals examined, a single PCR ribotype strain (type smz) was predominant and accounted for 22 (65%) of 34, 18 (64%) of 28, and 11 (44%) of 25 isolates, respectively. All of the 51 isolates that represented PCR ribotype smz were nontypeable by PFGE because of DNA degradation. Since the type smz strain did not react with any of the antisera against 10 different serogroups (A, B, C, D, F, G, H, I, K, and X), we prepared a new antiserum against a type smz isolate. All 51 type smz isolates presented identical banding patterns, reacting with the newly prepared antiserum (designated subserogroup JP-0 of serogroup JP). These results were compared with those of a strain from a hospital outbreak that occurred in New York, which has been identified as type J9 by restriction enzyme analysis and type 01/A by arbitrarily primed PCR but was nontypeable by PFGE because of DNA degradation. This strain was reported to be epidemic at multiple hospitals in the United States. The J9 strain represented a PCR ribotype pattern different from that of a type smz strain and was typed as subserogroup G-1 of serogroup G by immunoblot analysis. A single outbreak type causing nosocomial CDAD in Japan was found to be different from the strain causing multiple outbreaks in the United States, even though the outbreak strains from the two countries were nontypeable by PFGE because of DNA degradation.
机译：从 C患者中回收的艰难梭菌分离株。通过PCR分型，脉冲场凝胶电泳（PFGE）和Western免疫印迹这三种分型系统分析了日本不同地区三家医院的难治性腹泻（CDAD）。在接受检查的三家医院中，单一的PCR核型菌株（smz型）占主导地位，分别占34个菌株中的22个（65％），28个菌株中的18个（64％）和25个菌株中的11个（44％）。代表PCR核糖型smz的所有51个分离株均因DNA降解而无法通过PFGE分型。由于smz型菌株不会与针对10种不同血清群（A，B，C，D，F，G，H，I，K和X）的任何抗血清反应，因此我们制备了针对smz型分离株的新抗血清。所有51型smz分离株均呈现相同的条带模式，与新制备的抗血清（血清群JP的指定亚血清群JP-0）反应。将这些结果与纽约某医院爆发的菌株进行了比较，该菌株已通过限制性内切酶分析鉴定为J9型，通过任意引物PCR鉴定为01 / A型，但由于DNA降解无法通过PFGE鉴定。据报道该菌株在美国多家医院中流行。 J9菌株代表的PCR核糖核酸模式不同于smz菌株，通过免疫印迹分析被定为G血清型的G-1亚血清型。发现在日本引起医院内CDAD的单一暴发类型与在美国引起多次暴发的菌株不同，尽管由于DNA降解，两个国家的暴发菌株无法通过PFGE进行分型。

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