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首页> 外文期刊>Journal of Clinical Microbiology >PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron
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PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron

机译：日本分离的革兰氏阴性细菌携带的金属-β-内酰胺酶和整合子的遗传决定子的PCR分型，重点是3类整合子

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From January 2001 to December 2002, 587 strains of gram-negative bacterial isolates demonstrating resistance to ceftazidime and a combination of sulbactam and cefoperazone were subjected to a disk diffusion screening test using sodium mercaptoacetic acid; 431 strains (73.4%) appeared to produce metallo-β-lactamase (MBL). Of these 431 strains, 357 were found by PCR to carry genes for IMP-1 type MBL (blaIMP-1), while only 7 and 67 strains carried the IMP-2 gene (blaIMP-2) and the VIM-2 gene (blaVIM-2), respectively. Neither VIM-1 nor SPM-1 type MBL genes were found among the strains tested. Of 431 strains, 427 carried the intI1 gene, and 4 strains carrying both the intI1 and intI3 genes were reidentified as Pseudomonas putida harboring blaIMP-1. Of these four P. putida strains, three strains and one strain, respectively, were separately isolated from two hospitals located in the same prefecture, and the three strains showed very similar pulsed-field gel electrophoresis patterns. Of 357 blaIMP-1 carriers, 116, 53, 51, 47, and 30 strains were identified as Pseudomonas aeruginosa, Alcaligenes xylosoxidans, P. putida/fluorescens, Serratia marcescens, and Acinetobacter baumannii, respectively. Four strains carrying blaIMP-2 were reidentified as P. putida. Sixty-three P. aeruginosa strains and four P. putida strains carried blaVIM-2. Of 427 intI1-positive strains, 180, 53, 51, 47, and 35 were identified as P. aeruginosa, A. xylosoxidans, P. putida/fluorescens, S. marcescens, and A. baumannii, respectively. In the present study, it was confirmed that strains carrying blaIMP-1 with a class 1 integron are the most prevalent type in Japan, although several intI3 carriers have also been identified sporadically in this country.
机译：从2001年1月至2002年12月，对587株对头孢他啶具有抗药性的革兰氏阴性细菌菌株及舒巴坦和头孢哌酮的组合进行了巯基乙酸钠的圆盘扩散筛选试验。 431株（73.4％）似乎产生金属β-内酰胺酶（MBL）。通过PCR发现这431株菌株中，有357株携带IMP-1型MBL基因（ bla IMP-1 ），而只有7株和67株携带IMP- 2个基因（ bla IMP-2 ）和VIM-2基因（ bla VIM-2 ） 。在测试的菌株中均未发现VIM-1和SPM-1型MBL基因。在431个菌株中，有427个带有 intI1 基因，而同时携带 intI1 和 intI3 基因的4个菌株被重新标识为 Pseudomonas putida < / em>带有 bla IMP-1 。在这四个 P中。从位于同一州的两家医院分别分离出恶臭菌株，三种菌株和一种菌株，这三种菌株的脉冲场凝胶电泳图谱非常相似。在357个 bla IMP-1 携带者中，有116、53、51、47和30个菌株被鉴定为绿脓杆菌，产碱菌木糖氧化酶， P。分别是putida / fluorescens ，粘质沙雷氏菌（Serratia marcescens ）和鲍曼不动杆菌（emcinetobacter baumannii）。携带 bla IMP-2 的四个菌株被重新鉴定为 P。恶臭。六十三分铜绿菌菌株和四个 P。 putida 菌株携带 bla VIM-2 。在427个 intI1 阳性菌株中，有180、53、51、47和35个被鉴定为 P。铜绿， A。木糖氧化酶， P。 putida / fluorescens ， S。 marcescens 和 A。鲍曼氏菌。在本研究中，已证实携带 bla IMP-1 且具有1类整合子的菌株是日本最流行的菌株，尽管有几种 intI3

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