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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis of Microbial Diversity in Advanced Caries
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Molecular Analysis of Microbial Diversity in Advanced Caries

机译：晚期龋病中微生物多样性的分子分析

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Real-time PCR analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. This suggests that an unresolved complexity exists in bacteria associated with advanced caries. In this report, the profile of the microflora of carious dentine was explored by using DNA extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of Prevotella spp. Using universal primers for the 16S rRNA gene, PCR amplicons were cloned, and approximately 100 transformants were processed for each lesion. Phylogenetic analysis of 942 edited sequences demonstrated the presence of 75 species or phylotypes in the 10 carious lesions. Up to 31 taxa were represented in each sample. A diverse array of lactobacilli were found to comprise 50% of the species, with prevotellae also abundant, comprising 15% of the species. Other taxa present in a number of lesions or occurring with high abundance included Selenomonas spp., Dialister spp., Fusobacterium nucleatum, Eubacterium spp., members of the Lachnospiraceae family, Olsenella spp., Bifidobacterium spp., Propionibacterium sp., and Pseudoramibacter alactolyticus. The mechanisms by which such diverse patterns of bacteria extend carious lesions, including the aspect of infection of the vital dental pulp, remain unclear.
机译：对晚期龋病病变中细菌总数的实时PCR分析表明，细菌总数超过了可培养细菌的数量。这表明与晚期龋齿有关的细菌中存在未解决的复杂性。在本报告中，通过使用从10个病灶中提取的DNA提取龋齿牙本质的微生物谱，该10个病灶是根据可比较的总微生物载量和 Prevotella spp的相对丰度选择的。使用针对16S rRNA基因的通用引物，克隆PCR扩增子，并为每个病变处理大约100个转化子。对942个编辑序列的系统进化分析表明，在10个龋齿病变中存在75种或系统型。每个样本中最多代表31个分类单元。发现各种各样的乳酸杆菌占该物种的50％，而原核生物也丰富，占该物种的15％。存在于许多病灶中或高丰度的其他分类单元包括 Selenomonas spp。， Dialister spp。， Fusobacter nucleatum ， Eubacterium spp。， Lachnospiraceae 家族的成员， Olsenella spp。，双歧杆菌 spp。， Propionibacterium 菌和乳酸假单胞菌。这种细菌多样化形态扩展龋齿病变的机制（包括重要牙髓感染方面）的机制仍不清楚。

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