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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Identification of Capnocytophaga spp. via 16S rRNA PCR-Restriction Fragment Length Polymorphism Analysis
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Molecular Identification of Capnocytophaga spp. via 16S rRNA PCR-Restriction Fragment Length Polymorphism Analysis

机译：Capnocytophaga spp的分子鉴定。 16S rRNA PCR-限制性片段长度多态性分析

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Capnocytophaga spp. have been implicated as putative periodontal pathogens associated with various periodontal diseases. Although the genus is known to contain five human oral isolates, accurate identification to species level of these organisms recovered from subgingival plaque has been hampered by the lack of a reliable method. Hence, most studies to date have reported these isolates as Capnocytophaga spp. Previous attempts at identification were based on biochemical tests; however, the results were inconclusive. Considering the differing virulence features of the respective isolates, it is crucial to identify these isolates to species level. The universal and conservative nature of the 16S rRNA gene has provided an accurate method for bacterial identification. The aim of this study was to identify Capnocytophaga spp. via restriction enzyme analysis of this gene (16S rRNA PCR-restriction fragment length polymorphism). The results (backed up by 16S rRNA gene sequencing) showed that this method reliably identifies all named Capnocytophaga spp. to species level.
机译： Capnocytophaga spp。已经被认为是与各种牙周疾病相关的推定牙周病原体。尽管已知该属包含五种人类口腔分离株，但是由于缺乏可靠的方法而无法准确鉴定从龈下斑块中回收的这些生物的物种水平。因此，迄今为止，大多数研究已将这些分离物报告为 Capnocytophaga spp。先前的鉴定尝试是基于生化测试;但是，结果尚无定论。考虑到各个分离株的毒力特征不同，在物种水平上鉴定这些分离株至关重要。 16S rRNA基因的普遍性和保守性为细菌鉴定提供了一种准确的方法。这项研究的目的是确定 Capnocytophaga spp。通过对该基因进行限制性内切酶分析（16S rRNA PCR限制性片段长度多态性）。结果（得到16S rRNA基因测序的支持）表明，该方法能够可靠地鉴定所有命名的 Capnocytophaga spp。到物种水平。

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