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首页> 外文期刊>Journal of Clinical Microbiology >Characteristics of Staphylococcus aureus Strains Isolated in Poland in 1996 to 2004 That Were Deficient in Species-Specific Proteins
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Characteristics of Staphylococcus aureus Strains Isolated in Poland in 1996 to 2004 That Were Deficient in Species-Specific Proteins

机译：1996年至2004年在波兰分离的缺乏物种特异性蛋白的金黄色葡萄球菌菌株的特征

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One hundred seventy Staphylococcus aureus isolates, collected in 1996 to 2004, were reidentified by phenotypic and genotypic methods. One hundred ten of these (65%) were confirmed, as previously denoted, to be clumping factor (CF)- or free coagulase-deficient S. aureus, based on their phenotype. Based on the CF or coagulase production, three groups of phenotypically deficient S. aureus isolates were distinguished. Group 1 encompassed CF-positive and coagulase-deficient isolates, group 2 consisted of CF-deficient and coagulase-positive isolates, and group 3 included isolates that were CF positive, had delayed coagulase activity, and were deficient in other species-specific features. All investigated strains harbored the clfA, clfB, coa, spa, and nuc genes, but the presence of their products was not detected by the phenotypic methods. Glycopeptide susceptibility testing showed that 26 isolates (23.6%) were hetero-glycopeptide-intermediate S. aureus(hGISA) or hetero-teicoplanin-intermediate S. aureus (hTISA), based on the population analysis profile. The relatedness of the isolates was evaluated by multiple-locus variable number of tandem repeats analysis, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. The phenotypically deficient S. aureus isolates were classified into PFGE types B (ST239-III) and D (ST246-IA) and were related to the common clones, Hungarian and Iberian, respectively, which have been widely disseminated in Poland and globally. The simultaneous occurrence of hGISA/hTISA and the CF-deficient phenotypes was found for 62.1% of isolates belonging to group 2. The majority of these isolates were assigned to the Iberian clone (PFGE type D; ST247-IA). An association between the defect in coagulase and that in thermonuclease production was observed, which concerned 59.2% of isolates of group 1. The majority of these isolates belonged to the Hungarian clone (PFGE type B; ST239-III).
机译：通过表型和基因型方法对1996年至2004年收集的170株金黄色葡萄球菌进行了鉴定。如前所述，其中一百零一（65％）被确认为聚集因子（CF）或游离凝结酶缺陷型 S。金黄色葡萄球菌，基于它们的表型。根据CF或凝固酶的产生，三组表型缺陷的 S。分离出金黄色葡萄球菌。第1组包括CF阳性和凝固酶缺陷型分离株，第2组由CF缺乏和凝固酶阳性分离株组成，第3组包括CF阳性，凝固酶活性延迟和其他物种特异性特征不足的分离株。所有调查的菌株均含有 clfA ， clfB ， coa ， spa 和 nuc 基因，但其表型方法未检测到其产物的存在。糖肽敏感性测试显示26个分离株（23.6％）是杂糖肽中间体 S。金黄色葡萄球菌（hGISA）或异替考拉宁中间产物 S。金黄色葡萄球菌（hTISA），基于人口分析资料。通过多位点可变数目的串联重复分析，脉冲场凝胶电泳（PFGE）和多位点序列分型来评估分离株的相关性。表型缺陷的 S。金黄色葡萄球菌分离株分为PFGE B型（ST239-III）和D型（ST246-IA），分别与常见的克隆匈牙利和伊比利亚有关，已在波兰和全球广泛传播。对于属于第2组的62.1％的分离株，发现同时发生hGISA / hTISA和CF缺陷表型。这些分离株中的大多数被归入伊比利亚克隆（PFGE类型D; ST247-IA）。观察到凝固酶缺陷与热核酸酶生产缺陷之间的关联，涉及19.2组的59.2％分离株。这些分离株中的大多数属于匈牙利克隆（PFGE B型; ST239-III）。

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