Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing

Michael J. Lodes; Dominic Suciu; Mark Elliott; Axel G. Stover; Marty Ross; Marcelo Caraballo; Kim Dix; James Crye; Richard J. Webby; Wanda J. Lyon; David L. Danley; Andrew McShea

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Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing

机译：基于半导体的寡核苷酸微阵列用于甲型流感病毒亚型的鉴定和测序

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In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.

机译：面对对流感大流行的担忧，必须迅速获得对强毒A型流感病毒的鉴定，以做出有效反应。然而，即使在装备精良的病毒学实验室中，快速亚型的鉴定也很困难，或者在更严酷的条件下无法在现场获得。在这里，我们描述了一种可用于快速鉴定甲型流感病毒血凝素亚型1至15和神经氨酸酶亚型1至9的基因组测定和微阵列设计。还描述了可用于对两个基因的部分序列进行测序的基于酶的测定或其他感兴趣的序列。

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《Journal of Clinical Microbiology》 |2006年第4期|共10页
作者
Michael J. Lodes; Dominic Suciu; Mark Elliott; Axel G. Stover; Marty Ross; Marcelo Caraballo; Kim Dix; James Crye; Richard J. Webby; Wanda J. Lyon; David L. Danley; Andrew McShea;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray [J] . Phenix-Lan Quan, Gustavo Palacios, Omar J. Jabado, Journal of Clinical Microbiology . 2007,第8期

机译：GreeneChipResp寡核苷酸微阵列检测呼吸道病毒和甲型流感病毒的亚型。
2. Subtype identification of the novel A H1N1 and other human influenza A viruses using an oligonucleotide microarray. [J] . Kang X, Li Y, Sun H, Archives of virology . 2010,第1期

机译：使用寡核苷酸微阵列鉴定新型A H1N1和其他人类甲型流感病毒的亚型。
3. Subtype identification of the novel A H1N1 and other human influenza A viruses using an oligonucleotide microarray [J] . Xiaoping Kang, Yongqiang Li, Honghe Sun, Archives of Virology . 2010,第1期

机译：使用寡核苷酸微阵列鉴定新型A H1N1和其他人类甲型流感病毒的亚型
4. Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay [C] . Bagus Nanang Luwito, Ahmad Nadif, Retno D. Soejoedono, International Conference in One Health . 2018

机译：南苏拉威病南苏拉威病株中孤立的禽流感病毒亚禽流感病毒的亚型鉴定，血凝集抑制作用
5. Molecular detection and identification of avian influenza viruses by cDNA microarray. [D] . Maughan, Michele Nancy. 2007

机译：通过cDNA芯片对禽流感病毒进行分子检测和鉴定。
6. Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing [O] . Michael J. Lodes, Dominic Suciu, Mark Elliott, 2006

机译：基于半导体的寡核苷酸微阵列用于甲型流感病毒亚型的鉴定和测序
7. Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing [O] . Lodes, Michael J., Suciu, Dominic, Elliott, Mark, 2006

机译：基于半导体的寡核苷酸微阵列用于甲型流感病毒亚型的鉴定和测序
8. Universal Detection and Identification of Avian Influenza Virus by Use of Resequencing Microarrays [R] . 2009

机译：利用重测序微阵列对禽流感病毒的通用检测与鉴定

Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing

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