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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of New Variable-Number Tandem-Repeat Systems for Typing Mycobacterium tuberculosis with Beijing Genotype Isolates from Beijing, China
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Evaluation of New Variable-Number Tandem-Repeat Systems for Typing Mycobacterium tuberculosis with Beijing Genotype Isolates from Beijing, China

机译：用来自北京的北京基因型分离株鉴定结核分枝杆菌的新型可变数目串联重复系统的评价

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The newly proposed variable-number tandem-repeat (VNTR) typing system, which includes a basic 15-locus set and a high-resolution 24-locus set (P. Supply et al., J. Clin. Microbiol. 44:4498-4510, 2006), demonstrated a high power for the discrimination of Mycobacterium tuberculosis isolates collected worldwide. To evaluate its ability to differentiate the Beijing genotype strains from the Beijing area in China, 72 isolates with typical Beijing or Beijing-like spacer oligonucleotide typing profiles were subjected to typing with the VNTR system (24 loci) and typing by restriction fragment polymorphism analysis with IS6110 (IS6110-RFLP). Compared to the “old” 12-locus VNTR typing method, use of the 15- and 24-locus systems had a dramatically improved power to discriminate the Beijing genotype strains. A subtle difference in the Hunter-Gaston discriminatory index (HGI) between the 15-locus and the 24-locus systems resulted from only one locus, Mtub29. However, the VNTR-based clusters could be further differentiated by IS6110-RFLP (HGI by IS6110 RFLP, 0.999), although in one case an IS6110 cluster was subdivided by the 15-locus VNTR system. In this sense, use of the newly proposed 15-locus VNTR system along with the Mtub29 locus can serve as a first-line typing method for the epidemiological study of M. tuberculosis isolates in Beijing, while secondary typing of clustered strains by IS6110-RFLP is still required.
机译：新提出的可变数目串联重复（VNTR）分型系统，包括一个基本的15位基因组和一个高分辨率的24位基因组（P. Supply等人，J。Clin。Microbiol。44：4498- 4510，2006）证明了对全世界收集的结核分枝杆菌菌株的高分辨力。为了评估其区分中国北京地区北京基因型菌株的能力，对具有典型北京或北京样间隔区寡核苷酸分型特征的72个分离株进行了VNTR系统分型（24个位点），并通过限制性片段多态性分析进行分型。 IS 6110 （IS 6110 -RFLP）。与“旧的” 12个位点的VNTR分型方法相比，使用15和24个位点的系统可以显着提高辨别北京基因型菌株的能力。 15个位点和24个位点的系统之间的Hunter-Gaston鉴别指数（HGI）的细微差别仅由一个位点Mtub29引起。但是，可以通过IS 6110 -RFLP进一步区分基于VNTR的群集（IS 6110 RFLP的HGI，0.999），尽管在一种情况下是IS 6110 集群由15个位置的VNTR系统细分。从这个意义上讲，新提出的15位VNTR系统与Mtub29基因座的结合可以用作 M流行病学研究的一线分类方法。结核病在北京分离，而仍需要通过IS 6110 -RFLP对集簇菌株进行二次分型。

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