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首页> 外文期刊>Journal of Clinical Microbiology >Use of Variable-Number Tandem-Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping
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Use of Variable-Number Tandem-Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping

机译：利用可变数目串联重复分型区分香港的北京分枝杆菌和与IS6110限制性片段长度多态性分型和寡核苷酸分型的比较

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摘要

The Mycobacterium tuberculosis Beijing family isolates may cause more than a quarter of all tuberculosis cases worldwide, are emerging in some areas, and are often associated with drug resistance. Early recognition of transmission of this genotype is therefore important. To evaluate the usefulness of variable-number tandem-repeat (VNTR) typing to discriminate and recognize strains of the Beijing family, M. tuberculosis isolates from Hong Kong were subjected to VNTR analysis, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. The allelic diversity of the 14 VNTR loci included in the analysis varied from 0 to 0.618 among Beijing strains. The discriminatory power of VNTR analysis was slightly lower than that of IS6110 RFLP. Our analysis shows that VNTR typing, which has many practical advantages over RFLP typing, can be used for epidemiological studies of Beijing strains. However, VNTR-defined clusters should be subtyped with IS6110 RFLP for maximal resolution.
机译：结核分枝杆菌北京分离株可能导致全世界所有结核病例的四分之一以上，在某些地区正在出现，并且通常与耐药性有关。因此，早期识别这种基因型的传播很重要。为了评估可变数目串联重复（VNTR）分型来区分和识别北京家族株的有用性， M。对来自香港的结核病菌株进行VNTR分析，血吸虫分型和IS 6110 限制性片段长度多态性（RFLP）分型。分析中包括的14个VNTR基因座的等位基因多样性在北京菌株中从0到0.618不等。 VNTR分析的判别力略低于IS 6110 RFLP。我们的分析表明，VNTR分型比RFLP分型具有许多实际优势，可以用于北京菌株的流行病学研究。但是，为获得最大分辨率，应使用IS 6110 RFLP对VNTR定义的簇进行亚型化。

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